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Overexpression of glutathione S-transferase pi messenger RNA and its relationship to gene amplification in head and neck squamous cell carcinoma

  • Oncology
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Abstract

Human glutathione S-transferase pi has been known to be a good marker for several tumor types because of the high frequency with which it is overexpressed. In order to determine whether GST pi is useful as an indicator for head and neck cancers, expression of GST pi was investigated by Northern analysis. Overexpression of mRNA was detected in 9 of 36 primary head and neck squamous cell carcinomas. To examine the relationship between overexpression and amplification of GST pi gene, Southern analysis was performed on all samples. Only 3 of the 36 tumors showed amplification GST pi genes, indicating that gene amplification may not play a key role in GST pi mRNA overexpression in these cancers.

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References

  1. Batist G, Tulpule A, Sinha BK, Kathki AG, Myers CE, Cowan KH (1986) Overexpression of a novel anionic glutathione transferase in multidrug-resistant human breast cancer cells. J Biol Chem 261:15544–15549

    PubMed  CAS  Google Scholar 

  2. Davis LG, Giber MD, Batte JF (1986) Basic methods in molecular biology. Elsevier, New York

    Google Scholar 

  3. Hayes JD, Mantle TJ (1986) Use of immunoblot techniques to discriminate between the glutathione S-transferase Yf, Yk, Yn/Yb and Yc subunits and to study their extra-hepatic distribution. Biochem J 233:779–788

    PubMed  CAS  Google Scholar 

  4. Hiratsuka A, Sebata N, Kawashima K, Okuda H, Ogura K, Watabe T, Satoh K, Hatayama I, Tsuchida S, Ishikawa T, Sato K (1990) A new class of glutathione transferase Yrs-Yrs inactivating reactive sulfate esters as metabolites of carcinogenic arylmethanols. J Biol Chem 265:11973–11981

    PubMed  CAS  Google Scholar 

  5. Ishioka C, Kanamaru R, Shibata H, Konishi Y, Ishikawa A, Wakui A, Sato T, Nishihira T (1991) Expression of glutathione S-transferase π messenger RNA in human esophageal cancers. Cancer 67:2560–2564

    Article  PubMed  CAS  Google Scholar 

  6. Jacoby WB (1978) The glutathione-S transferases: a group of multifunctional detoxification proteins. Adv Enzymol 46:383–414

    Google Scholar 

  7. Jares P, Fernandez PL, Campo E, Nadel A, Bosch F, Aiza G, Nayach I, Traserra J, Cardesa A (1994)PRAD1/cyclin D1 gene amplification correlates with messenger RNA overexpression and tumor progression in human laryngeal carcinomas. Cancer Res 54:4813–4817

    PubMed  CAS  Google Scholar 

  8. Kano T, Sakai M, Muramatsu M (1987) Structue and expression of a human class π glutathione S-transferase messenger RNA. Cancer Res 47:5626–5630

    PubMed  CAS  Google Scholar 

  9. Ketterer B (1986) Detoxification reactions of glutathione and glutathione transferases. Xenobiotica 16:957–973

    Article  PubMed  CAS  Google Scholar 

  10. Kodate C, Fukushi A, Narita T, Kudo H, Soma Y, Sato K (1986) Human placental form of glutathione S-transferase (GST-π) as a new immunohistochemical marker for human colonic carcinoma. Gann 77:226–229

    PubMed  CAS  Google Scholar 

  11. Lundqvist G, Yucel-lindberg T, Morgenstern R (1992) The oligomeric structure of rat liver microsomal glutathione transferase studied by chemical cross-linking. Biochim Biophys Acta 1159:103–108

    PubMed  CAS  Google Scholar 

  12. Mannervik B, Alin P, Guthenberg C, Jenson H, Tahir MK, Warholm M, Jornvall H (1985) Identification of three classes of cytosolic glutathione transferase common to several mammalian species: correlation between structural data and enzymatic properties. Proc Natl Acad Sci USA 82:7202–7206

    Article  PubMed  CAS  Google Scholar 

  13. Mannevuk B (1985) The isozyme of glutathione transferase. Adv Enzymol 57: 357–417

    Google Scholar 

  14. Meyer DJ, Coles B, Pemble SE, Gilmore KS, Fraser GM, Ketter B (1991) Theta, a new class of glutathione transferase purified from rat and man. Biochem J 274:409–414

    PubMed  CAS  Google Scholar 

  15. Morrow CS, Chin J, Cowan KH (1992) Posttranscriptional control of glutathione S-transferase π gene expression in human cancer cells. J Biol Chem 267:10544–10550

    PubMed  CAS  Google Scholar 

  16. Moscow JA, Fairchild CR, Madden MJ, Ransom DT. Wieand HS, O'Brien EE, Poplack DG, Cossman J, Myers CE, Cowan KH (1989) Expression of anionic glutathione-S-transferase and p-glycoprotein genes in human tissues and tumors. Cancer Res 49:1422–1428

    PubMed  CAS  Google Scholar 

  17. Mulder TPJ, Verspaget HW, Sier CFM, Roelofs HMJ, Ganesh S, Griffioen G, Peters WHM (1995) Glutathione S-transferase π in colorectal tumors is predictive for overall survival. Cancer Res 55:2696–2702

    PubMed  CAS  Google Scholar 

  18. Ogura K, Nishiyama T, Okada T, Kajita J, Narihata H, Watabe T, Hiratsuka A, Watabe T (1991) Molecular cloning and amino acid sequencing of rat liver class theta glutathione S-transferase Yrs-Yrs inactivating reactive sulfate esters of carcinogenic arylmethanols. Biochem Biophys Res Commun 181:1294–1300

    Article  PubMed  CAS  Google Scholar 

  19. Pickett CB, Lu AYH (1989) Glutathione S-transferases: gene structure, regulation and biological function. Annu Rev Biochem 58:743–764

    Article  PubMed  CAS  Google Scholar 

  20. Sambrook J, Fritsh EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY

    Google Scholar 

  21. Satoh K, Kitahara A, Soma Y, Inaba Y, Hatayma I, Sato K (1985) Purification, induction and distribution of placental glutathione transferase: a new marker enzyme for preneoplastic cells in rat chemical hepatocarcinogenesis. Proc Natl Acad Sci USA 82:3964–3968

    Article  PubMed  CAS  Google Scholar 

  22. Shea TC, Kelly SL, Henner WD (1988) Identification of an anionic form of glutathione transferase present in human tumors and cell lines. Cancer Res 48:527–533

    PubMed  CAS  Google Scholar 

  23. Shiratori Y, Soma Y, Maruyama H, Sato SD, Takano A, Sato K (1987) Immunohistochemical detection of the placental form glutathione S-transferase in dysplastic and neoplastic human uterine cervix lesions. Cancer Res 47:6806–6809

    PubMed  CAS  Google Scholar 

  24. Suzuki T, Coggan M, Shaw DC, Board PG (1987) Electrophoretic and immunological analysis of human glutathione S-transferase isozymes. Ann Hum Genet 51:95–99

    PubMed  CAS  Google Scholar 

  25. Tsuchida S, Sato K (1992) Glutathione transferases and cancer. CRC Crit Rev Biochem Mol Biol 27:337–384

    CAS  Google Scholar 

  26. Tsutsumi M, Sugisaki T, Makino T, Miyagi N, Nakani K, Shiratori T (1987) Oncofetal expression of glutathione S-transferase placental form in human stomach carcinoma. Gann 78:631–633

    PubMed  CAS  Google Scholar 

  27. Wang X, Pavelic ZP, Li Y, Wang L, Gleich L, Gluckman JL, Stambrook PJ (1995) Amplification and overexpression of the human cyclin D1 gene in head and neck squamous cell carcinoma. J Clin Pathol Mol Pathol 48: M256-M259

    Article  Google Scholar 

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Authors and Affiliations

  1. Department of Otolaryngology-Head and Neck Surgery, College of Medicine, University of Cincinnati, 45267, Cincinnati, OH, USA

    Z. P. Pavelic, Y. Li, L. Gleich & J. L. Gluckman

  2. Division of Molecular Oncology, Department of Otolaryngology, Head and Neck Surgery, University of Cincinnati, College of Medicine, 231 Bethesda Avenue, 45267-0528, Cincinnati, OH, USA

    Z. P. Pavelic

  3. Department of Cell Biology, College of Medicine, University of Cincinnati, 45267, Cincinnati, OH, USA

    X. Wang & P. J. Stambrook

Authors
  1. Z. P. Pavelic
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  2. X. Wang
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  3. Y. Li
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  4. L. Gleich
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  5. J. L. Gluckman
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  6. P. J. Stambrook
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Pavelic, Z.P., Wang, X., Li, Y. et al. Overexpression of glutathione S-transferase pi messenger RNA and its relationship to gene amplification in head and neck squamous cell carcinoma. Eur Arch Otorhinolaryngol 254 (Suppl 1), S144–S146 (1997). https://doi.org/10.1007/BF02439746

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