Biotechnology Techniques

, Volume 6, Issue 3, pp 245–248 | Cite as

Affinity purification of ribonuclease T1 on 5′-AMP-sepharose

  • S. U. Gite
  • V. Shankar
Article

Summary

A simple and rapid procedure, involving heat treatment followed by chromatography on Bio-Gel P-10 and 5′-AMP-Sepharose, was developed for the purification of RNase T1, from commercially available Takadiastase powder with an overall yield of 67%. The purified enzyme was electrophoretically homogeneous.

Keywords

Affinity Purification Aspergillus Oryzae Affinity Matrix Rapid Procedure National Chemical Laboratory 

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Copyright information

© Science & Technology Letters 1992

Authors and Affiliations

  • S. U. Gite
    • 1
  • V. Shankar
    • 1
  1. 1.Division of Biochemical SciencesNational Chemical LaboratoryPuneIndia

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