Biotechnology Techniques

, Volume 6, Issue 3, pp 193–198 | Cite as

Use of flow cell reactors to quantify biofilm formation kinetics

  • Ching-Tsan Huang
  • Steven W. Peretti
  • James D. Bryers


A parallel plate flow cell reactor is introduced and used to evaluate cell adhesion and biofilm formation kinetics for four different bacterial strains of the species,E. coli. The reactor allows biofilm growth under defined, well-controlled fluid dynamics while providing continuous observations and direct sampling of biofilm for biological, chemical and physical analyses as well as immunofluorescent labeling.


Chemical Oxygen Demand Chemostat Culture Capillary Reactor Fresh Nutrient Solution Kinetic Growth Parameter 
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  1. Atkinson, B. (1986) In:Process Engineering Aspects of Immobilized Cell Systems, C. Webb, G.M. Black, and B. Atkinson, eds., Inst. Chem. Eng. Rugby, Warwickshire.Google Scholar
  2. Bakke, R. (1986). “Biofilm Detachment”, Ph.D. Dissertation, Montana State University, Bozeman, MTGoogle Scholar
  3. Bakke, R. and Olsson, P.W. (1986),J. Microbiol. Methods, 5:93–98.CrossRefGoogle Scholar
  4. Bryers, J.D. (1987). Biotechnology Process, 3:57–68.CrossRefGoogle Scholar
  5. Caldwell, D.E. and Lawence, J.R. (1988) In:CRC Handbook of Laboratory Model Systems for Microbial Ecosystems, vol. 1, J.W.T. Wimpenny, pp. 117–138., Florida: CRC PressGoogle Scholar
  6. Characklis, W.G. and Marshall, K.C., eds. (1990),Biofilms, New York: John Wiley & Sons, Inc.Google Scholar
  7. Escher, A.R. (1986), “Bacterial colonization of a smooth surface: An analysis with image analyzer”, Ph.D. Dissertation, Montana State University, Bozeman, MTGoogle Scholar
  8. Huang, C.T. and Bryers, J.D. (1991), submitted toBiofouling Google Scholar
  9. McCoy, W.F., Bryers, J.D., Robbins, J., and Costerton, J.W. (1981),Can. J. Microbiol., 27:910–917.CrossRefGoogle Scholar
  10. Pickup, R.W. and Saunders, J.R. (1990)Trends in Biotechnology, 8: 329–335.CrossRefGoogle Scholar
  11. Powell, M.S. and Slater, N.K.H. (1983),Biotechnol. Bioengrg., 25: 891–900.CrossRefGoogle Scholar
  12. Robison, D.K. and Wang, D.I.C. (1986) “A Novel Bioreactor System for Biopolymer Production”, presented at Biochemical Engineering V., Engineering Foundation Conference, Henniker, NHGoogle Scholar
  13. Sjollema, J., Busscher, H.J., and Weerkamp, A.H. (1988)Biofouling 1:101–112Google Scholar
  14. Trulear, M.G. (1983), “Cellular reproduction and extracellular polymer formation in the development of biofilms”, Ph.D. Dissertation, Montana State University, Bozeman, MTGoogle Scholar
  15. Turakhia, M.H. (1986), “The influence of calcium on biofilm processes”, Ph.D. Dissertation, Montana State University, Bozeman, MTGoogle Scholar
  16. Zimmerman, R. and Meyer-Reil, L. (1974)Kiel. Meeresforsch., 30: 24–27Google Scholar

Copyright information

© Science & Technology Letters 1992

Authors and Affiliations

  • Ching-Tsan Huang
    • 1
  • Steven W. Peretti
    • 2
  • James D. Bryers
    • 1
  1. 1.The Center for Biochemical EngineeringDuke UniversityDurhamUSA
  2. 2.Department of Chemical EngineeringNorth Carolina State UniversityRaleighUSA

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