Biotechnology Techniques

, Volume 6, Issue 3, pp 193–198 | Cite as

Use of flow cell reactors to quantify biofilm formation kinetics

  • Ching-Tsan Huang
  • Steven W. Peretti
  • James D. Bryers
Article

Summary

A parallel plate flow cell reactor is introduced and used to evaluate cell adhesion and biofilm formation kinetics for four different bacterial strains of the species,E. coli. The reactor allows biofilm growth under defined, well-controlled fluid dynamics while providing continuous observations and direct sampling of biofilm for biological, chemical and physical analyses as well as immunofluorescent labeling.

Keywords

Chemical Oxygen Demand Chemostat Culture Capillary Reactor Fresh Nutrient Solution Kinetic Growth Parameter 

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Copyright information

© Science & Technology Letters 1992

Authors and Affiliations

  • Ching-Tsan Huang
    • 1
  • Steven W. Peretti
    • 2
  • James D. Bryers
    • 1
  1. 1.The Center for Biochemical EngineeringDuke UniversityDurhamUSA
  2. 2.Department of Chemical EngineeringNorth Carolina State UniversityRaleighUSA

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