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A newly isolatedBacillus stearotheromophilus K1041 and its transformation by electroporation

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Biotechnology Techniques

Summary

Optimal conditions for the plasmid transformation of a newly isolatedBacillus stearothermophilus K1041 by electroporation were investigated. The optimal conditions allowed a transformation efficiency of 5.8×105 transformants per μg plasmid pUB110.

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Authors and Affiliations

  1. Department of Agricultural Chemistry, Utsunomiya University, 350 Minemachi, 321, Utsunomiya, Tochigi, Japan

    Issay Narumi, Kazumi Sawakami & Tadashi Yanagisawa

  2. NEC corporation, 4-1-1 Miyazaki, Miyamae-ku, 216, Kawasaki, Kanagawa, Japan

    Shinya Nakamoto & Noriyuki Nakayama

  3. The Institute of Physical and Chemical Research, 2-1 Hirosawa, 351-01, Wako, Saitama, Japan

    Nobutaka Takahashi

  4. Jichi Medical School, School of Nursing, 3311-159 Yakushiji, 329-04, Minamikawachi, Tochigi, Japan

    Hiroshi Kihara

Authors
  1. Issay Narumi
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  2. Kazumi Sawakami
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  3. Shinya Nakamoto
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  4. Noriyuki Nakayama
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  5. Tadashi Yanagisawa
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  6. Nobutaka Takahashi
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  7. Hiroshi Kihara
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Narumi, I., Sawakami, K., Nakamoto, S. et al. A newly isolatedBacillus stearotheromophilus K1041 and its transformation by electroporation. Biotechnol Tech 6, 83–86 (1992). https://doi.org/10.1007/BF02438695

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  • DOI: https://doi.org/10.1007/BF02438695

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