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Enzymatic peptide fragment condensation. Choice of reaction media for the synthesis of an insect neuropeptide derivative

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Biotechnology Techniques

Summary

The insect neuropeptide derivative Z-Gly-Gly-Ser-Leu-Tyr-Ser-Phe-Gly-Leu-NH2 has been obtained in good yields by condensation of two peptide fragments through formation of the Tyr5-Ser6 bond by α-chymotrypsin (α-CT). Among the three reaction media examined, reversed micelles and low water containing acetonitrile, but not aqueous-N,N dimethyl-formamide (DMF) solutions, proved to be suitable systems to assist α-chymotrypsin catalysis.

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Authors and Affiliations

  1. Unit for Peptide Chemistry and Biochemistry, CID-CSIC, Jordi Girona 18-26, E-08034, Barcelona, Spain

    N. Xaus, X. Jorba, S. Calvet, P. Clapés, J. L. Torres & G. Valencia

  2. Department of Organic Chemistry, University of Barcelona, Martí i Franquès 1-11, E-08028, Barcelona, Spain

    F. Albericio

Authors
  1. N. Xaus
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  2. X. Jorba
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  3. S. Calvet
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  4. F. Albericio
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  5. P. Clapés
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  6. J. L. Torres
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  7. G. Valencia
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Xaus, N., Jorba, X., Calvet, S. et al. Enzymatic peptide fragment condensation. Choice of reaction media for the synthesis of an insect neuropeptide derivative. Biotechnol Tech 6, 69–72 (1992). https://doi.org/10.1007/BF02438692

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  • DOI: https://doi.org/10.1007/BF02438692

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