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Characterization of rifamycin oxidase immobilized on nylon fibers

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Biotechnology Techniques

Summary

Rifamycin oxidase, an enzyme used in the biotransformation of rifamycin B to S was immobilized on nylon fibers using glutaraldehyde as the cross linking agent. An activity of 18 U/g of nylon fiber with a binding efficiency of 37% was achieved. The immobilized enzyme showed an operational stability of 7 days and was also protected against thermal inactivation. It exhibited a Km(app.) of 2.0mM.

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Authors and Affiliations

  1. Biochemical Engineering Research and Process Development Center, Institute of Microbial Technology, Sector 39-A, 160 014, Chandigarh, INDIA

    R. M. Vohra & V. V. Vyas

Authors
  1. R. M. Vohra
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  2. V. V. Vyas
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Vohra, R.M., Vyas, V.V. Characterization of rifamycin oxidase immobilized on nylon fibers. Biotechnol Tech 6, 61–64 (1992). https://doi.org/10.1007/BF02438690

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  • DOI: https://doi.org/10.1007/BF02438690

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