Biotechnology Techniques

, Volume 6, Issue 1, pp 53–54 | Cite as

Preparation of a nuclease-free alpha-amylase and its use in DNA purification

  • Stewart D. Finlayson
  • Fiona Bain
  • David R Berry
  • John R Johnston
Article
  • 24 Downloads

Summary

A thermostable alpha-amylase fromBacillus licheniformis when heated to 80°C for 20 min retains >90% of its activity but loses all DNAase activity. This allows the amylase to be used to purify yeast DNA.

Keywords

Amylase Amylase Activity Bacillus Licheniformis CsCl Density CsCl Density Gradient 

References

  1. Cryer, D.R., Eccleshall, R. and Marmur, J. (1975). In:Methods of Cell Biology, Prescott, D.M., ed. vol. XII. pp. 39–44, London: Academic Press.Google Scholar
  2. Davis, L.G., Dibner, M.D. and Battey, J.F. (1986).Basic Methods in Molecular Biology, p. 44, New York: Academic Press.Google Scholar
  3. Murray, M.G. and Thompson, W.F. (1980)Nucleic Acids Research 8, 4321–4325.Google Scholar

Copyright information

© Science & Technology Letters 1992

Authors and Affiliations

  • Stewart D. Finlayson
    • 1
  • Fiona Bain
    • 1
  • David R Berry
    • 1
  • John R Johnston
    • 1
  1. 1.Department of Bioscience and BiotechnologyUniversity of StrathclydeGlasgowUK

Personalised recommendations