Preparation of a nuclease-free alpha-amylase and its use in DNA purification
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A thermostable alpha-amylase fromBacillus licheniformis when heated to 80°C for 20 min retains >90% of its activity but loses all DNAase activity. This allows the amylase to be used to purify yeast DNA.
KeywordsAmylase Amylase Activity Bacillus Licheniformis CsCl Density CsCl Density Gradient
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© Science & Technology Letters 1992