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Rapid spectrophotometric determination of the exponential constant of ethanol-enhanced proton diffusion in yeasts

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Biotechnology Techniques

Summary

A rapid method for the determination of the exponential constant of ethanol-enhanced proton diffusion in yeasts has been developed. This method was based on Bromophenol Blue to monitor the acidification by energized yeast cells suspensions. This method is completely automated using a Beckman Biomek 1000 workstation.

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Authors and Affiliations

  1. Laboratoire de microbiologie et de technologie des fermentations, INRA-IPV, 2 place Viala, 34060, Montpellier Cedex 01, France

    J. C. Mauricio & J. M. Salmon

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  1. J. C. Mauricio
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  2. J. M. Salmon
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Mauricio, J.C., Salmon, J.M. Rapid spectrophotometric determination of the exponential constant of ethanol-enhanced proton diffusion in yeasts. Biotechnol Tech 6, 27–32 (1992). https://doi.org/10.1007/BF02438683

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  • DOI: https://doi.org/10.1007/BF02438683

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