Biotechnology Techniques

, Volume 5, Issue 4, pp 281–282 | Cite as

A simple and rapid method for selection of high diosgenin yielding clones ofDioscorea deltoidea callus

  • G. A. Ravishankar
  • S. Grewal
Article
  • 15 Downloads

Summary

Antimony pentachloride has been used to detect high diosgenin producing callus and cell clones ofDioscorea deltcidea grownin vitro. Using this method high yielding cultures, with a potential to produce up to 1.86% diosgenin, were selected.

Keywords

Diosgenin Tropane Tropane Alkaloid Central Food Technological Research Institute Antimony Pentachloride 

References

  1. Halasz, N.A., and Krehl, W.A. (1954) Yale J. Biol. Med. 27, 119–125.Google Scholar
  2. Heble, M.R., and Staba, E.J. (1980) Planta Med. Suppl. 124–128.CrossRefGoogle Scholar
  3. Kaul, B., and Staba, E.J. (1968) Lloydia 31, 171–179.Google Scholar
  4. Ravishankar, G.A., and Grewal, S. (1988) Curr. Sci. 57, 679–681.Google Scholar
  5. Ravishankar, G.A., and Grewal, S. (1990) Biochem. Intern. 20, 897–901.Google Scholar
  6. Ravishankar, G.A., and Grewal, S. (1991) Biotechnol. Lett. 13, 125–130.CrossRefGoogle Scholar
  7. Tal, B., Rokem, J.S., and Goldberg, I. (1983). Plant Cell Rep. 2, 219–222.CrossRefGoogle Scholar

Copyright information

© Science & Technology Letters 1991

Authors and Affiliations

  • G. A. Ravishankar
    • 1
  • S. Grewal
    • 2
  1. 1.Autotrophic Cell Culture DisciplineCentral Food Technological Research InstituteMysoreIndia
  2. 2.Regional Research LaboratoryPlant Tissue Culture SectionJammu-TawiIndia

Personalised recommendations