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An application of isoelectric focusing to non-human primates hair Keratin

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Human Evolution

Abstract

This study is an investigation on the Keratin of hair coming from 51 non-human Primates belonging to 11 families and 37 different species, using isoelectric focusing on thin layer of polyacrylmide gel (0.5 mm) in pH range 2.5–7.0 followed by silver staining.

Our results highlight that animals belonging to the same species have identical isoelectrophoretic patterns. It was proved forLemur fulvus, Macaca fascicularis, Cercopithecus aethiops, Gorilla gorilla.

Instead, regarding different species belonging to the same genus, we can say that the pattern obtained was not always characteristic of the species to which the protein belonged. In fact, whereas some species of theCercopithecus, Macaca andPapio genus show significantly different patterns, other species ofCercopithecus, Macaca, Papio, Cebus andSaimiri show identifical patterns. On the other hand, patterns belonging to different genus nearly always showed more marked differences. As for man, by means of this technique, it is possible to show a high number of polypeptides in the 3–4 pH range for non-human Primates as well.

Thus, species belonging to different families and genus can always be distinguished; on the other hand, it is not always possible for species of the same genus (e.g.Cebus, Papio).

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References

  • Budowle B. and Acton R.T., 1981.Isoelectric focusing of hair proteins. Proceedings of the 3rd international conference, Charleston, S.C., USA. Carracedo, A., Prieto, J.M., Concheiro, L.

  • Estefania J., 1987.Isoelectric focusing patterns of some mammalian keratins. Journal of Forensic Sciences, 32: 93–99.

    Google Scholar 

  • Chiarelli B., 1968.Taxonomy and phylogeny of old world Primates with references to the origin of man. Ed. Rosemberg & Sellier, Torino, pp. 323+XI. Proceedings of a round table held in Turin (Italy) on 3rd–6th June, 1967.

  • Chiarelli B., 1983.L'origine dell'uomo. Introduzione alla antropologia, Ed. Laterza, Roma-Bari, pp. 402+XIV.

    Google Scholar 

  • Gerhard M., 1987.Electrophoretic variability in human head hair: polyacrylamide gel electrophoresis of hair proteins in the presence of sodium dodecyl sulfate and urea. Electrophoresis, 8: 153–157.

    Article  Google Scholar 

  • Hrdy D.B. and Baden, H.P., 1973.Biochemical variation of hair keratins in man and non-human Primates. American Journal of Physical Anthropology, 39: 19–24.

    Article  Google Scholar 

  • Lindley H., et al., 1971. Cited by Marshall R.C., et al. 1977. Australian Journal of Zoology, 25: 121–132.

    Google Scholar 

  • Marshall R.C., Frenkel M.J., and Gillespie J.M., 1977.High-sulphur proteins in mammalian keratins: a possible aid in classification. Australian Journal of Zoology, 25: 121–132.

    Article  Google Scholar 

  • Marshall R.C., Gillespie J.M. and Klement, V., 1985.Methods and future prospects for forensic identification of hair by electrophoresis. Journal of Forensic Science Society, 25: 57–66.

    Article  Google Scholar 

  • Marshall R.C. and Williams K.M., 1986.High resolution two-dimensional electrophoresis of hair proteins without prior S-carboxymethilation. Electrophoresis, 7: 524–526.

    Article  Google Scholar 

  • Sarich V.M. and Wilson A.C., 1967.Immunological time scale for hominid evolution. Science, 158: 1200–1203.

    Google Scholar 

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Folin, M., Zanella, P. & Contiero, C. An application of isoelectric focusing to non-human primates hair Keratin. Hum. Evol. 8, 1–9 (1993). https://doi.org/10.1007/BF02436460

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