Abstract
Antioxygenic enzymes provide defense to the cell against free radicals produced during respiration. This study deals with the effect of propyl gallate feeding on antioxygenic and respiratory enzymes in liver of male mice of different age groups. Antioxidant increased the activities of catalase, glutathione reductase, ATPase and NADH-dehydrogenase significantly in 8-month-old mice, whereas the increase in activity in early and late growth stages was observed only at selective concentrations. The increased activities of antioxygenic enzymes on propyl gallate feeding may be attributed to an increase in the activities of respiratory enzymes.
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References
Halliwell, B.: Free radicals, oxygen toxicity and ageing, in: Age pigments, edited by Sohal, R.S., Elsevier/North Holland Biomed. Press, New York, 1981, pp. 1–62.
Allen, R.G., Farmer, K.J., and Sohal, R.S.: Effect of catalase inactivation on levels of inorganic peroxides, superoxide dismutase, glutathione, oxygen consumption and life span in adult houseflies (Musca domestica). Biochem. J., 216: 503–506, 1983.
Munkres, K.D., and Colvin, H.J.: Ageing of Neurospora crassa. II. Organic hydroperoxide toxicity and the protective role of antioxidant and the antioxygenic enzymes. Mech. Age. Develop., 5: 99–108, 1976.
Saprin, A.N., Kalinina, E.V., and Shulyakovskaya, T.S.: Effect of antioxidants on the activity of glutathione-s-transferase and sulfotransferases of the rat liver. Farmkol. Toksikol., 50: 81–84, 1987.
Ferreira, J.: Effect of butylated hydroxyanisole on electron transport in rat liver mitochondria. Biochem. Pharmacol., 40: 677–684, 1990.
Semsei, I., Rao, G., and Richardson, A.: Changes in the expression of superoxide dismutase and catalase as a function of age and dietary restrictions. Biochem. Biophys. Res, Commun., 164: 620–625, 1989.
Garg, S.K., Khanna, S.C., and Sharma, S.P.: Effect of antioxidants on adenosine triphosphatase activity of brain and heart in male mice during ageing. Ind. J. Exp. Biol., 30: 355–356, 1992.
Lee, S.S., Ye, J., Jones, D.P., and McCormick, D.B.: Correlation of H2O2 production and liver catalase during riboflavin deficiency and repletion in mammals. Biochem. Biophys. Res. Commun., 117: 788–793, 1983.
Gullapalli, S., Shivaswamy, V., Ramasarma, T., and Kurup, C.K.R.: Increase in α-glycerophosphate dehydrogenase and other oxido-reductase activities of hepatic mitochondria on administration of vanadate to the rat. Ind. J. Biochem. Biophys., 26: 227–233, 1989.
Roy, D., Pathak, D.N., and Singh, R.: Effect of centrophenoxine on the antioxidative enzymes in various regions of the rat brain. Exp. Gerontol., 18: 185–197, 1983.
Lints, F.A., and Lints, C.V.: Respiration in Drosophila. III. Influence of preimaginal environment on respiration and ageing in Drosophila melanogaster hybrids. Exp. Gerontol., 4: 81–94, 1969.
Dyundikova, V.A., Silvon, Z.K., and Dubina, T.L.: Biological age and its estimation. 1. Studies of some physiological parameters in albino rats and their validity as biological age tests. Exp. Gerontol., 16: 13–24, 1981.
Kar, M., and Mishra, D.B.: Catalase, peroxidase and polyphenoloxidase activities during rice leaf senescence. Plant Physiol., 57: 315–319, 1976.
Allen, R.G., Farmer, K.J., Newton, R.K., and Sohal, R.S.: Effect of paraquat administration on longevity, oxygen consumption, lipid peroxidation, superoxide dismutase, catalase, glutathione reductase, inorganic peroxides and glutathione in adult housefly. Comp. Biochem. Physiol., 78: 283–288, 1984.
Kielley, W.W.: Mg2+ activated muscle ATPase, in: Methods in Enzymology, Vol. 2, edited by Colowick, S.P., and Kaplan, N.O., Academic Press, New York, 1969, pp. 588–595.
Mackler, B.: Preparation and properties of soluble NADH-dehydrogenases from cardiac muscle, in: Methods in Enzymology, Vol. 10, edited by Estabrook, R.W., and Pullman, M.E., Academic Press, New York, 1967, pp. 275–294.
King, T.E.: Preparation of succinate dehydrogenase and reconstitution of succinate oxidase, in: Methods in Enzymology, Vol. 10, edited by Estabrook, R.W., and Pullman, M.E., Academic Press, New York, 1967, pp. 322–332.
Lowry, O.H., Rosenbrough, N.J., Farr, A.L., and Randall, R.J.: Protein measurement with Folinphenol reagent. J. Biol. Chem., 193: 265–275, 1951.
Khanna, S.C., Garg, S.K., and Sharma, S.P.: Antioxidant-influenced alterations in glutathione reductase activity in different age groups of male mice. Gerontol., 38: 9–12, 1992.
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Garg, S.K., Khanna, S.C. & Sharma, S.P. Effect of propyl gallate feeding on antioxygenic and respiratory enzymes in liver of mice. AGE 16, 93–95 (1993). https://doi.org/10.1007/BF02436140
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DOI: https://doi.org/10.1007/BF02436140