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Effect of Tulasi (Ocimum sanctum) leaf powder supplementation on blood sugar levels, serum lipids and tissues lipids in diabetic rats


Tulasi leaf powder was fed at the 1% level in normal and diabetic rats for a period of one month to explore the effect on fasting blood sugar, uronic acid, total amino acids, and the lipid profile in serum and tissue lipids. The results indicated a significant reduction in fasting blood sugar, uronic acid, total amino acids, total cholesterol, triglyceride, phospholipids and total lipids. In liver, total cholesterol, triglyceride and total lipids were significantly lowered. Total lipids were significantly reduced in kidney. In heart, a significant fall in total cholesterol and phospholipids was observed. All these observations indicate the hypoglycemic and hypolipidemic effect of Tulasi in diabetic rats.

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  1. 1.

    Grover GS, Rao JT (1977) Investigations on the antimicrobial efficiency of essential oils fromOcimum sanctum andOcimum gratissimum. Perfum Kosmet 58: 236.

    Google Scholar 

  2. 2.

    Reddy ACP, Lokesh BR (1994) Studies on the inhibitory effects of curcumin and eugenol on the formation of reactive oxygen species and the oxidation of ferrous iron. Mol Cell Biochem 137: 1–8.

    Article  CAS  Google Scholar 

  3. 3.

    Bhat JV, Broker R (1955) Anticoagulase factors in some indigenous plants. J Sci Ind Res 13: B: 305–306.

    Google Scholar 

  4. 4.

    Gonopoti RD (1954) Chemical composition ofOcimum sanctum. Cong. Luso-espanfarm 3:187–191.

    Google Scholar 

  5. 5.

    Trowell H (1977) Crude fiber, dietary fiber and atherosclerosis. Atherosclerosis 16: 138–140.

    Article  Google Scholar 

  6. 6.

    Anderson JW (1980) The role of dietary carbohydrate and fiber in control of diabetes. Adv Intern Med 26: 67–96.

    CAS  Google Scholar 

  7. 7.

    Mani UV, Goswamy S, Mani I, Patel S, Gujral S (1985) Effect of wheat-bran supplementation on serum lipids in diabetic rats. Arogya J Health Sci 11: 186–190.

    Google Scholar 

  8. 8.

    Mani I, Mani UV (1987) Effect of wheat-bran supplementation on blood sugar, glycosylated protein and serum lipids in NIDDM subjects. Plant Foods Hum Nutr 37: 161–168.

    Article  Google Scholar 

  9. 9.

    Mani UV, Sharma M, Waghray K, Iyer U, Mani I (1989) Effect of colocasia leaves on serum and tissue lipids in cholesterol fed rats. Plant Foods Hum Nutr 39: 244–255.

    Article  Google Scholar 

  10. 10.

    Iyer UM, Mehta NC, Mani I, Mani UV (1992) Studies on the effect of dry Sundakai (Solanum torvum) powder supplementation on lipid profile, glycated proteins and amino acids in non-insulin dependent diabetic patients. Plant Foods Hum Nutr 42: 175–182.

    Article  CAS  Google Scholar 

  11. 11.

    Iyer UM, Mani UV (1990) Studies on the effect of curry leaves supplementation (Murraya koenigii) on lipid profile, glycated proteins and amino acids in non-insulin dependent diabetic patients. Plant Foods Hum Nutr 40: 275–282.

    Article  CAS  Google Scholar 

  12. 12.

    Martinez et al. (1964) A possible hypoglycemic factor in Abahaca (Ocimum sanctum). Ch Pb 61: 4860-d. In: Pushpangaden P, Sobti SN (1977). Medicinal properties ofOcimum (Tulsi) species and some recent investigation of their efficacy. Indian Drugs Aug 77: 207–208.

  13. 13.

    Dhar ML, Dhar MM, Dhawan BN, Mehrotra BN, Ray C (1968) Screening of Indian plants for biological activity, Part I. Ind J Exp Biol 6: 232–247.

    CAS  Google Scholar 

  14. 14.

    Raghuramulu N, Nair KM, Kalyanasundaram S (1983) A Manual of Laboratory Techniques. National Institute of Nutrition, Hyderabad, India.

    Google Scholar 

  15. 15.

    Folch J, Lees M, Stanley GMS (1957) A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226: 497–509.

    CAS  Google Scholar 

  16. 16.

    Hultman E (1959) Method of Hultman E for blood sugar. Nature 183: 108–109.

    Article  CAS  Google Scholar 

  17. 17.

    Bitter T, Muir H (1962) A modified uronic acid carbazole reaction. Annal Biochem 4: 330–335.

    Article  CAS  Google Scholar 

  18. 18.

    Rosen H (1957) Modified ninhydrin colorimetric analysis for amino acids. Arch Biochem Biophys 67: 10–15.

    Article  CAS  Google Scholar 

  19. 19.

    Christopher K et al. (1972) Improved determination of total serum lipids by the sulphophospho vanillin reaction. Clin Chem 18: 673–674.

    Google Scholar 

  20. 20.

    Marinetti GV (1962) Chromatographic separation, identification and analysis of phosphatides. J Lipid Res 3: 1–4.

    CAS  Google Scholar 

  21. 21.

    Warnick GR et al. (1982) HDL-Cholesterol quantification by phosphotungstate Mg polyethylene glycol precipitation. Both with enzymatic cholesterol assay compared with the lipid research method. Am J Clin Pathol 78: 718–722.

    CAS  Google Scholar 

  22. 22.

    National Research Council (1972) Nutritional Requirements of the lab animals. National Academy Science Publication 277–278.

  23. 23.

    Truel B, Holestein CG, Fischer RN, Winternanter KH (1980) Non-enzymatic glycosylation of proteins. J Biol Chem 255: 6717–6720.

    Google Scholar 

  24. 24.

    Jenkins DJA et al. (1976) The cholesterol lowering properties of guar and pectin. Clin Sci Molec Med 51: 8–9.

    Google Scholar 

  25. 25.

    Sharma RD (1986) Evaluation of hypocholesterolemic factor of fenugreek seeds (Foenum graecum) in rats?. Nutr Rep Inter 33: 669–676.

    CAS  Google Scholar 

  26. 26.

    Sharma RD (1984) Hypocholesterolemic activity of some Indian gums. Nut Res 4: 381–389.

    Article  CAS  Google Scholar 

  27. 27.

    Khan BA, Abraham A, Leelamma S (1995) Hypoglycemic effect ofMurraya koenigii andBrasica juncea: mechanism of action. Ind J Biochem Biophys 32: 106–108.

    CAS  Google Scholar 

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Correspondence to U. V. Mani.

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Rai, V., Iyer, U. & Mani, U.V. Effect of Tulasi (Ocimum sanctum) leaf powder supplementation on blood sugar levels, serum lipids and tissues lipids in diabetic rats. Plant Food Hum Nutr 50, 9–16 (1997).

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Key words

  • Diabetes mellitus
  • Tulasi leaves
  • Serum and tissue lipids