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Rate of excretion of vitamin C in human urine

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Abstract

The dosage of vitamin C necessary to maintain a level in the urine which could be detected using the 2,6-dichlorophenolindophenol assay was determined with undergraduate students. Students taking 250 mg daily did not excrete significant levels of vitamin C in their urine, while excretion increased at doses from 0.5 to 2 g. A 2 g daily dose caused detectable excretion from about 4 until 16 hr later, on both the first and eighth day. A dose of 500 mg taken every 12 hr led to continuously-detectable levels of vitamin C in the urine. The conclusion is that two conditions are necessary to elevate vitamin c excretion continuously: a dose of at least 500 mg and a dose every 12 hr. This is substantially higher than the U.S. recommended daily allowance and more frequent than administration being used in clinical trials.

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References

  1. Davies, M.B., Austin, J., and Partridge, D.A.: Vitamin C: Its Chemistry and Biochemistry. Cambridge, Royal Society of Chemistry, 1991, pp. 7–25.

    Google Scholar 

  2. Stare, F.J., and Stare, I.M.: Charles Glen King, 1896–1988. J. Nutr., 118:1272–7, 1988.

    PubMed  CAS  Google Scholar 

  3. Roig, M.G., Rivera, Z.S., and Kennedy, J.F.: L-ascorbic acid: an overview. Int. J. Food. Sci. Nutr., 44:59–72, 1993.

    CAS  Google Scholar 

  4. Block, G.: Vitamin C, Cancer and Aging. Age, 16:55–8, 1993.

    CAS  Google Scholar 

  5. Marwick, C.: Cancer institute takes a look at ascorbic acid. JAMA, 264:1926, 1990.

    Article  PubMed  CAS  Google Scholar 

  6. Wittes, R.E.: Vitamin C and Cancer. New Engl. J. Med., 312-178–9, 1985.

    Article  PubMed  CAS  Google Scholar 

  7. Burr, M.L., Bares, C.J., and Godberg, G.: Incidence for premature rupture of membranes in pregnant women with low leukocyte levels of vitamin C. Eur. J. Clin. Nutr., 39c:387–8, 1985.

    Google Scholar 

  8. Kimura, H., Yamada, Y., and Morita, Y.: Dietary ascorbic acid depresses plasma and low density lipoprotein lipid peroxidation in genetically scorbutic rats. J. Nutr., 122:1904–9, 1992.

    PubMed  CAS  Google Scholar 

  9. Uchida, K., Nomura, U., and Takase, H.: Effect of vitamin C depletion on serum cholesterol and lipoprotein levels in ODS (od/od) rats unable to synthesize ascorbic acid. J. Nutr., 120:1140–7, 1990.

    PubMed  CAS  Google Scholar 

  10. Blanchard, J., Conrad, K.A., and Watson, R.R.: Comparison of plasma, mononuclear and polymorphonuclear leukocyte vitamin C levels in young and elderly women during depletion and supplementation. Eur. J. Clin. Nutr., 43: 97–106, 1989.

    PubMed  CAS  Google Scholar 

  11. Chavance, M., Herveth, B., and Fournier, C.: Vitamin status, immunity and infections in an elderly population. Eur. J. Clin. Nutr., 43:827–35, 1989.

    PubMed  CAS  Google Scholar 

  12. Vallance, S.: Platelets, Leukocytes and buffy layer vitamin C after surgery. Hum. Nutr., 40c:35–41, 1986.

    Google Scholar 

  13. Vojdani, A., and Ghoneum, M.: In vivo effect of ascorbic acid enhancement of human natural killer cell activity. Nutr. Res., 13:753–1993.

  14. Pryor, W.A.: The formation of free radicals and the consequences of their reactions in vivo. Photochem. Photobiol., 28:787–801, 1978.

    PubMed  CAS  Google Scholar 

  15. Harman, D.: The aging process. Proc. Natl. Acad. Sci. USA, 78:7124–7128, 1981.

    PubMed  CAS  Google Scholar 

  16. Tappel, A.L: Vitamin E as the biological lipid antioxidant. Vitam. Horm., 20:493–510, 1962.

    Article  CAS  Google Scholar 

  17. Niki, E., Saito, T., Kawakami, A., and Kamiya, Y.: Inhibition of oxidation of methyl-linoleate in solution by vitamin E and vitamin C. J. Biol. Chem., 259:4177–4182, 1984.

    PubMed  CAS  Google Scholar 

  18. Harman, D.: Free Radical Theory of Aging: Current Status, in Lipofuscin-1987: State of the Art, edited by Zs.-Nagy, I., New York, Elsevier, 1988, pp. 3–21.

    Google Scholar 

  19. Gussow, J.D., and Thomas, P.: Nutrition Debate: Sorting Out Some Answers, Menlo Park, CA, Bull Pub., 1986.

    Google Scholar 

  20. Pauling, L.: Vitamin C and the Common Cold, New York, Bantam Books, 1970.

    Google Scholar 

  21. Griffith, H.W.: Complete Guide to Vitamins, Minerals, and Supplements, Tucson, AZ, Fisher Books, 1988, p. 2.

    Google Scholar 

  22. Levine, M.: New concepts in the biology and biochemistry of ascorbic acid. New Engl. J. Med., 314: 892–902, 1986.

    Article  PubMed  CAS  Google Scholar 

  23. Levine, M., Cantillena, C.C., and Dhariwal, K.R.: In situ kinetics and ascorbic acid requirements. World Rev. Nutr. Diet., 72:114–27, 1993.

    PubMed  CAS  Google Scholar 

  24. Olson, J.A., and Hodges, R.E.: The scientific basis of the suggested new RDA values for vitamins A and C. Nutr. Today, 20:14–15, 1985.

    Google Scholar 

  25. Blanchard, J., Conrad., K.A., and Garry, P.J.: Effects of age and intake on vitamin C disposition in females. Eur. J. Clin. Nutr., 44:447–460, 1990.

    PubMed  CAS  Google Scholar 

  26. Omaye, T.S., Turnbull, J.D., and Sauberlich, H.E.: Selected Methods for the Determination of Ascorbic Acid in Animal Cells, in Methods Enzymol.: Vitamins and Coenzymes, Vol. 62, edited by McCormick, D.B., and Wright, L.D., New York, Academic Press, 1979, pp. 3–11.

    Google Scholar 

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King, G., Beins, M., Larkin, J. et al. Rate of excretion of vitamin C in human urine. AGE 17, 87–92 (1994). https://doi.org/10.1007/BF02435011

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