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Glutathione levels in hepatic and extrahepatic tissues of mice as a function of age

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Abstract

Glutathione is the most abundant thiol-containing component in living cells and is believed to play an important role as an antioxidant. We have examined the levels of reduced, oxidized, and total glutathione in liver, blood, kidneys, and intestinal mucosa of mice as a function of age. Reduced glutathione levels decreased in kidneys, Intestinal mucosa, and blood while total glutathione levels decreased in all tissue with advanced age. Highest concentrations of reduced glutathione per μg protein were present in liver and intestinal mucosa. Our results support the hypothesis that a decrease in reduced glutathione may contribute to changes associated with aging as well as to the increased susceptibility to disease processes which occur with advanced age.

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References

  1. Harman, D.: The Biology of Aging: Possible Role of Free Radical Reactions. Bios. 46: 74–83, 1975.

    Google Scholar 

  2. Goldstein, S.: The Biology of Aging: New Eng. J. Med. 285:1120–1129, 1971.

    CAS  Google Scholar 

  3. Timiras, P. S.: Biological Perspectives on Aging. Amer. Scientist 66: 605–613, 1978.

    CAS  Google Scholar 

  4. Harman, D.: Prolongation of the Normal Life Span by Radiation Protection Chemicals. J.Geront. 12: 275–261, 1957.

    Google Scholar 

  5. Harman, D.: Prolongation of the Normal Life Span and Inhibition of Spontaneous Cancer by Antioxidants. J. Geront. 16: 247–254, 1961.

    PubMed  CAS  Google Scholar 

  6. Harman, D.: Free Radical Theory of Aging: Effect of Free Radical Reaction Inhibitors on the Mortality Rate of Male LAF1Mice. J. Geront. 23: 476–482, 1968.

    PubMed  CAS  Google Scholar 

  7. Harman, D.: Free Radical Theory of Aging: Effect of Free Radical Reaction Inhibitors on the Life Span of Male LAF Mice — Second Experiment. The Gerontol. 8:III: 13, 1968.

    Google Scholar 

  8. Comfort, A. Youhototsky-Gore, I. and Pathmenathan, K.: Effect of Ethoxyquin on the Longevity of C3H Mice. Nature, 229: 254–255, 1971.

    Article  PubMed  CAS  Google Scholar 

  9. Kohn, R. R.: Effect of Antioxidants on Life Span of C57BL Mice. J. Geront. 26: 378–380, 1971.

    CAS  Google Scholar 

  10. Harman, D., Heidrick, M. L. and Eddy, D. E.: Free Radical Theory of Aging: Effect of Free-Radical-Reaction Inhibitors on the Immune Response. J. Amer. Ger. Soc. 25: 400–407, 1977.

    CAS  Google Scholar 

  11. Aries, I. M. and Jakoby, W. B., eds. Glutathione: Metabolism and Function, Raven Press, New York, 1976, pp. 1–34.

    Google Scholar 

  12. Harman, D.: Aging: A Theory Based on Free Radical and Radiation Chemistry. J. Geront., 11: 298–300, 1956.

    PubMed  CAS  Google Scholar 

  13. Leot, S., Yiengst, M. and Barrows, C. H.: The Effect of Age on the Sulfhydryl Content of Serum Albumin. The Gerontol. 8:III; 13, 1968.

    Google Scholar 

  14. Bertolini, A. M., Quarto di Palo, F. M. and Spinnler, H. R.: Variations in Erythrocytic Sulfhydryl Groups in Relation to Individual Age, and During Physiological Aging. Acta Gerontol. 12: 1–7, 1962.

    Google Scholar 

  15. Goldschmidt, L.: Variations in Red Cell Glutathione with Aging in Male and Female Primaquine-Sensitive Negroes. Clin. Biochem. 4: 34–37, 1971.

    Article  PubMed  CAS  Google Scholar 

  16. Eng, L. I. L., Wan, W. P. and Ng, T.: Reduced Glutathione, Glutathione Reductase, Glutathione Peroxide, and Pyruvate Kinase in Erythrocytes of Human Newborns and Adults in Malaysia. Brit. J. Haematol. 25: 577–583, 1973.

    CAS  Google Scholar 

  17. Smith, J. D.: Blood Glutathione in Aging Lambs. Amer. J. Vet. Res. 34: 591–592, 1973.

    PubMed  CAS  Google Scholar 

  18. Lambert, G. H. and Thorgiersson, S. S.: Glutathione in the Developing Mouse Liver. 1. Developmental Curve and Depletion after Acetaminophen Treatment. Biochem. Pharmacol. 25: 1777–1781, 1976.

    Article  PubMed  CAS  Google Scholar 

  19. Pinto, R. E. and Bartley, W.: The Effect of Age and Sex on Glutathione Reductase and Glutathione Peroxidase Activities and on Aerobic Glutathione Oxidation in Rat Liver Homogenates. Biochem. J. 112: 109–115, 1969.

    PubMed  CAS  Google Scholar 

  20. Hissin, P. J. and Hilf, R.: A Fluorometric Method for the Determination of Oxidized and Reduced Glutathione in Tissues. Anal. Biochem. 74: 214–226, 1976.

    Article  PubMed  CAS  Google Scholar 

  21. Lowry, O. H., Rosebrough, N. J., Farr, A. L. and Randall, R. J.: Protein Measurement with the Folin Phenol Reagent. J. Biol. Chem. 193: 265–275, 1951.

    PubMed  CAS  Google Scholar 

  22. Meyers, J. L. Fundamentals of Experimental Design, 3rd, Ed., Allyn and Bacon, Inc., Boston, pp 90–91, 1978.

    Google Scholar 

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Authors and Affiliations

  1. Department of Biomedicinal Chemistry, University of Nebraska Medical Center, Omaha, Nebraska, 68105

    S. J. Stohs, J. M. Hassing, W. A. Al-Turk & A. N. Masoud

  2. Department of Anesthesiology, University of Nebraska Medical Center, Omaha, Nebraska, 68105

    A. N. Masoud

Authors
  1. S. J. Stohs
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  2. J. M. Hassing
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  3. W. A. Al-Turk
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  4. A. N. Masoud
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Stohs, S.J., Hassing, J.M., Al-Turk, W.A. et al. Glutathione levels in hepatic and extrahepatic tissues of mice as a function of age. AGE 3, 11–14 (1980). https://doi.org/10.1007/BF02435000

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