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One-step sandwich enzyme immunoassay for serum myoglobin with three monoclonal antibodies to different epitopes

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Abstract

Enzyme-linked sandwich immunosorbent assay for serum myoglobin with 3 monoclonal antibodies to different epitopes is described. Antibodies 13G6 and 18H2 were adsorbed on a carrier, and 14D6 antibodies were labeled with horseradish peroxidase. One-step incubation with standard myoglobin solution yielded double sandwich: adsorbed antibodies—myoglobin—labeled antibodies; the intensity of peroxidase reaction was directly proportional to myoglobin concentration. The sensitivity of the proposed method for serum myoglobin was below 5 ng/ml. The sensitivity of the method increases due to myoglobin binding with two antibodies adsorbed on the carrier. Fluctuations within and between series did not exceed 10%.

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Authors and Affiliations

  1. Laboratory of Clinical Neuroimmunology, Center of Psychic Health, Russian Academy of Medical Sciences, Moscow

    V. A. Nikulina & G. I. Kolyaskina

  2. Laboratory of Neurochemistry of Conditioned Reflex, Institute Higher Nervous Activity and Neurophysiology, Russian Academy of Sciences, Moscow

    E. A. Kizim, Yu. S. Massino, O. L. Segal, M. B. Smirnova & A. D. Dmitriev

  3. Association of Medical Laboratory Diagnostics, Moscow

    V. V. Avilov, D. B. Saprygin & S. P. Smotrov

Authors
  1. V. A. Nikulina
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  2. E. A. Kizim
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  3. Yu. S. Massino
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  4. O. L. Segal
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  5. M. B. Smirnova
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  6. V. V. Avilov
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  7. D. B. Saprygin
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  8. S. P. Smotrov
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  9. G. I. Kolyaskina
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  10. A. D. Dmitriev
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Additional information

Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 127, No. 5, pp. 597–600, May, 1999

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Nikulina, V.A., Kizim, E.A., Massino, Y.S. et al. One-step sandwich enzyme immunoassay for serum myoglobin with three monoclonal antibodies to different epitopes. Bull Exp Biol Med 127, 544–546 (1999). https://doi.org/10.1007/BF02434963

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  • DOI: https://doi.org/10.1007/BF02434963

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