Detection of cytomegalovirus DNA, viral antigens, and antibodies to it in patients after organ transplantation

  • M. S. Dolgikh
  • A. M. Vedyakov
  • E. Yu. Feoktistova
  • S. M. Adueva
  • N. E. Makarova
  • A. A. Kushch
  • B. Pustovoit
Virology
  • 22 Downloads

Abstract

Cytomegalovirus (CMV) DNA was determined by the PCR method, anti-CMV IgM and IgG were analyzed by enzyme immunoassay, and infective activity of CMV was evaluated by the rapid culture technique with monoclonal antibodies in the blood of patients, who underwent organ transplantation. In cases when the results coincided, PCR was much more sensitive, while the rapid culture technique was more reliable in predicting the course and outcome of CMV disease. Enzyme immunoassay failed to detect active infection by the moment of examination.

Key Words

organ transplantation cytomegalovirus (CMV) infection PCR diagnosis anti-CMV IgM infective activity of CMV 

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Copyright information

© Kluwer Academic/Plenum Publishers 1990

Authors and Affiliations

  • M. S. Dolgikh
    • 1
  • A. M. Vedyakov
    • 1
  • E. Yu. Feoktistova
    • 1
  • S. M. Adueva
    • 2
  • N. E. Makarova
    • 2
  • A. A. Kushch
    • 2
  • B. Pustovoit
    • 3
  1. 1.Institute of Transplantology and Artificial OrgansMinistry of Health of the Russian FederationMoscow
  2. 2.D. I. Ivanovsky Institute of VirologyRussian Academy of Medical SciencesMoscow
  3. 3.Institute of VirologyUniversity of LeipzigGermany

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