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Mechanisms of modulation of GABA-induced currents in isolated cerebellar cells by tacrine

  • Biophysics and Biochemistry
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Bulletin of Experimental Biology and Medicine Aims and scope

Abstract

Tacrine, when applied by the concentration clamp technique to isolated Purkinje cells from rat cerebellum, dose-dependently reduced the amplitude of GABA-activated chloride currents recorded by the patch clamp technique. Half-maximal inhibition (IC50) of currents activated by 2 μM GABA was observed at a tacrine concentration of 107 μM. Tacrine produced a right shift of the dose-response curves for GABA-induced currents without affecting their peak amplitudes. It is suggested that suppression of GABA-induced currents caused by tacrine can not be attributed to its interaction with the benzodiazepine site of the GABAA receptor.

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Authors and Affiliations

  1. Brain Research Institute, Russian Academy of Medical Sciences, Moscow

    S. N. Kolbaev, I. N. Sharonova, V. S. Vorob'ev & V. G. Skrebitskii

Authors
  1. S. N. Kolbaev
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  2. I. N. Sharonova
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  3. V. S. Vorob'ev
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  4. V. G. Skrebitskii
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Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 127, No. 5, pp. 539–542, May, 1999

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Kolbaev, S.N., Sharonova, I.N., Vorob'ev, V.S. et al. Mechanisms of modulation of GABA-induced currents in isolated cerebellar cells by tacrine. Bull Exp Biol Med 127, 485–488 (1999). https://doi.org/10.1007/BF02434947

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  • DOI: https://doi.org/10.1007/BF02434947

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