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Antioxidant properties of lactoferrin from human milk

  • S. Belizi
  • I. A. Nazarova
  • I. A. Klimova
  • V. N. Prokof'ev
  • N. V. Pushkina
Biophysics and Biochemistry

Abstract

Chemiluminescent methods showed that human lactoferrin more effectively inhibits free radical processes in the Fenton reaction than histidine and mannitol, usual free radical scavengers. Human lactoferrin added to yolk lipoprotein suspension in the presence of rhodamine 6G reduces the intensity of fast flash of Fe2+-induced chemiluminescence by 37%. Complete saturation of lactoferrin with iron reduces its antioxidant properties by 15.4%, the intensity of chemiluminescence being below the control by 25.7%.

Key Words

lactoferrin apolactoferrin hydrogen peroxide chemiluminescence free-radical reactions 

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References

  1. 1.
    Yu. V. Vladimirov, O. A. Azizova, A. I. Deev,et al., Biofizika,29, 250 (1991).Google Scholar
  2. 2.
    V. A. Gusev, O. S. Brusov, and L. F. PanchenkoVopr. Med. Khimii,26, No. 3, 291–301 (1980).PubMedGoogle Scholar
  3. 3.
    S. N. Efuni, E. Ya. Kaplan, A. I. Lukash,et al., Byull. Eksp. Biol. Med.,98, No. 7, 42–43 (1984).Google Scholar
  4. 4.
    I. Ya. Kon' and K. S. Ladodo,Pediatriya., No. 2, 17 (1992).Google Scholar
  5. 5.
    V. D. Ott, S. V. Dyukareva, and O. R. Mel'nikov,Vopr. Pitaniya No. 1, 79 (1993).Google Scholar
  6. 6.
    M. P. Sherstnev,Vopr. Med. Khimii,36, No. 1, 19–21 (1990).Google Scholar
  7. 7.
    R. I. Yakubovskaya, E. R. Nemtsova, and I. D. Kokhanova, —Ibid.,,32, No. 6, 75–79 (1986).Google Scholar
  8. 8.
    J. V. Bannister, W. H. Bannister, and H. A. Hill,Biochim. Biophys. Acta,715, No. 1, 116–120 (1982).PubMedGoogle Scholar
  9. 9.
    H. Kawakami, M. Hiratsuka, Sh. Dosako,Biol. Chem.,52, No. 4, 903–908 (1988).Google Scholar
  10. 10.
    J. Tenovuo, Z. Moldoveanu, J. Mestechy,et al., J. Immunol.,128, No. 2, 726–731 (1982).PubMedGoogle Scholar

Copyright information

© Kluwer Academic/Plenum Publishers 1999

Authors and Affiliations

  • S. Belizi
    • 1
    • 2
  • I. A. Nazarova
    • 1
    • 2
  • I. A. Klimova
    • 1
    • 2
  • V. N. Prokof'ev
    • 1
    • 2
  • N. V. Pushkina
    • 1
    • 2
  1. 1.Department of Biochemistry and MicrobiologyRostov State UniversityRostov-on-Don
  2. 2.Institute of MicrobiologyEpidemiology, and HygieneRostov-on-Don

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