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Bulletin of Experimental Biology and Medicine

, Volume 129, Issue 2, pp 136–139 | Cite as

Separation of peripheral polypeptides from soybean lipid extracts and their effects on structural organization of phospholipids

  • O. M. Martynova
  • G. M. Sorokoumova
  • A. A. Selishcheva
  • S. G. Alekseeva
  • O. P. Tyurina
  • V. I. Shvets
Biophysics and Biochemistry

Abstract

Fluorescence assay of soybean lipid extract showed the presence of polypeptides with tryptophan residues in hydrophobic and polar microenvironment. The peripheral polypeptide with polar tryptophan can be separated from phospholipids in solution with high ionic strength.31P nuclear magnetic resonance spectroscopy demonstrated that this polypeptide stabilized the phospholipid bilayer.

Key Words

soybean phospholipids polypeptides fluorescence magnetic resonance spectroscopy 

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Copyright information

© Kluwer Academic/Plenum Publishers 2000

Authors and Affiliations

  • O. M. Martynova
    • 1
  • G. M. Sorokoumova
    • 1
  • A. A. Selishcheva
    • 2
  • S. G. Alekseeva
    • 1
  • O. P. Tyurina
    • 2
  • V. I. Shvets
    • 1
  1. 1.M. V. Lomonosov Academy of Fine Chemical TechnologyMoscowUSSR
  2. 2.Biological FacultyM. V. Lomonosov Moscow State UniversityMoscowUSSR

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