Radiation and Environmental Biophysics

, Volume 35, Issue 2, pp 95–99 | Cite as

Changes in cellular proteins ofDeinococcus radiodurans followingγ-irradiation

  • Atsushi Tanaka
  • Hisashi Hirano
  • Masahiro Kikuchi
  • Shigeru Kitayama
  • Hiroshi Watanabe
Original Paper

Abstract

In order to examine radiation-induced proteins in an extremely radioresistant bacterium,Deinococcus radiodurans R 1, changes in cellular proteins after γ-irradiation were analysed by two-dimensional gel electrophoresis and silver staining. Nine proteins (190, 120, 87, 60, 58, 52, 46, 41 and 41 kDa) were increased (or appeared) and more than 13 proteins diminished after γ-irradiation at 6 kGy. Increase of eight proteins (except for 190-kDa protein) was prevented when the cells were irradiated in the presence of chloramphenicol. Three proteins, 87, 60 and 46 kDa, continued to be synthesized during post-irradiation incubation, and the amounts of these proteins increased with higher doses in a range of 1–12 kGy. Changes in the amount of proteins after irradiation in theR 1 strain were compared with those in a moderately radioresistant mutant (rec 1) and in a highly radiosensitive mutant (rec30). These three proteins were increased in bothR 1 andrec 1, but not inrec 30, suggesting that they are characteristic for radioresistant strains. In addition, from the microsequence analysis, the 46-kDa protein was found to be homologous to the EF-Tu protein ofEscherichia coli, whereas the remarkable homologous sequence to the N-terminal of the 60-kDa protein was not found among the known proteins.

Keywords

Deinococcus Radiodurans Japan Atomic Energy Research Institute Unirradiated Cell Thymine Glycol Winged Bean Seed 

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Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • Atsushi Tanaka
    • 1
    • 2
  • Hisashi Hirano
    • 3
  • Masahiro Kikuchi
    • 1
    • 2
  • Shigeru Kitayama
    • 4
  • Hiroshi Watanabe
    • 1
    • 2
  1. 1.Biotechnology LaboratoryTakasaki Radiation Chemistry Research EstablishmentJapan
  2. 2.Atomic Energy Research InstituteTakasaki, GunmaJapan
  3. 3.Department of Molecular BiologyNational Institute of Agrobiological ResourcesTsukuba, IbarakiJapan
  4. 4.Cellular Physiology LaboratoryThe Institute of Physical and Chemical Research (RIKEN), WakoSaitamaJapan

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