Bulletin of Experimental Biology and Medicine

, Volume 128, Issue 5, pp 1109–1111 | Cite as

Effects of thymidine and phorbol 12-myristate 13-acetate on erythroid differentiation of K562 cells and their sensitivity to nonspecific lysis by rat splenocytes

  • A. G. Anisimov
  • I. A. Bolotnikov
  • T. O. Volkova
Biophysics and Biochemistry


Induction of hemoglobin synthesis in K562 cells by thymidine (2 mM)in vitro did not significantly enhance major histocompatibility complex antigen-unrestricted lysis of splenocyte-exposed K562 cells. Inhibition of thymidine-induced hemoglobin synthesis by simultaneous incubation of cells with thymidine and phorbol 12-myristate 13-acetate (100 nM) decreased cytolytic activity of splenocytes against K562 cells. Preincubation of tumor cells with phorbol ester alone did not affect major histocompatibility complex antigen-unrestricted lysis induced by rat splenocytes but decreased the basal level of hemoglobin synthesis.

Key Words

nonspecific cytotoxicity erythroid differentiation phorbol 12-myristate 13-acetate 


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Copyright information

© Kluwer Academic/Plenum Publishers 2000

Authors and Affiliations

  • A. G. Anisimov
    • 1
  • I. A. Bolotnikov
    • 1
  • T. O. Volkova
    • 1
  1. 1.Department of BiochemistryPetrozavodsk State UniversityPetrozavodskUSSR

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