In Vivo oligonucleotide-protein interactions in the blood
- 23 Downloads
The interaction of oligonucleotides with serum proteins was studiedin vivo by the method of affinity modification. Alkylating oligonucleotide derivatives administered to animals induced the formation of oligonucleotide-protein complexes circulating in the blood for a long-time. Oligonucleotides in these complexes were partially protected from nucleases. The major oligonucleotide-binding proteins identified with specific antibodies were albumins and IgG. In the erythrocyte fraction no specific oligonucleotide-binding proteins were detected, while membrane-cytosolic leukocyte fraction contained an oligonucleotide-binding protein with a molecular weight of 72 kD.
Key Wordsoligonucleotides Affinity modification serum proteins immunoglobulins albumins
Unable to display preview. Download preview PDF.
- 1.N. V. Amirkhanov and V. F. Zarytova,Bioorgan. Khimiya,21, 365–375 (1995).Google Scholar
- 2.V. V. Vlasov, L. V. Pautova, E. Yu. Rykova, and L. A. Yakubov,Biokhimiya,58, 1247–1251 (1993).Google Scholar
- 3.V. F. Zarytova, E. M. Ivanova, and I. V. Kutyavin,Bioorgan. Khimiya,8, 224–230 (1982).Google Scholar
- 4.L. V. Pautova, E. Yu. Rykova, P. P. Laktionov, and V. V. Vlasov,Molekul. Biol.,30, 941–950 (1996).Google Scholar
- 9.Handbook of Biochemistry and Molecular Biology of Nucleic Acids. Ed. T. E. Fasman, Vol. 1, Cleveland (1975).Google Scholar
- 12.E. Yu. Rykova, P. P. Laktionov, and V. V. Vlasov,Nucleosides Nucleotides,16, 1883–1891 (1997).Google Scholar