In Vivo oligonucleotide-protein interactions in the blood
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The interaction of oligonucleotides with serum proteins was studiedin vivo by the method of affinity modification. Alkylating oligonucleotide derivatives administered to animals induced the formation of oligonucleotide-protein complexes circulating in the blood for a long-time. Oligonucleotides in these complexes were partially protected from nucleases. The major oligonucleotide-binding proteins identified with specific antibodies were albumins and IgG. In the erythrocyte fraction no specific oligonucleotide-binding proteins were detected, while membrane-cytosolic leukocyte fraction contained an oligonucleotide-binding protein with a molecular weight of 72 kD.
Key Wordsoligonucleotides Affinity modification serum proteins immunoglobulins albumins
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