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A comparative analysis of radiometric and autoradiographic methods for studies of DNA-synthesizing activities of cellsin vitro

  • D. S. Gembitskii
  • A. I. Antokhin
  • N. Ya. Popova
  • Yu. A. Romanov
  • Yu. N. Podyusov
Methods
  • 16 Downloads

Abstract

The DNA synthesis in cultured Ehrlich ascites carcinoma cells was studied by autoradiography and liquid-scintillation radiometry. The kinetics of total radioactivity of incorporated3H-thymidine correlated with the kinetics of the index of radioactivity. The radiometric assay provided an extremely precise estimation of changes in the DNA synthesisin vitro. In several cases the autoradiographic assay was more sensitive. We assumed that autoradiography and liquid-scintillation radiometry are interchangeable methods for studies of DNA synthesisin vitro. These assays are recommended for studying the effects of cell proliferation inhibitors on DNA synthesis.

Key Words

autoradiography Ehrlich ascites tumor liquid-scintillation radiometry proliferation inhibitors DNA synthesis 

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Copyright information

© Kluwer Academic/Plenum Publishers 1999

Authors and Affiliations

  • D. S. Gembitskii
    • 1
  • A. I. Antokhin
    • 1
  • N. Ya. Popova
    • 1
  • Yu. A. Romanov
    • 1
  • Yu. N. Podyusov
    • 1
  1. 1.Department of BiologyMedical and Biological Faculty of the Russian State Medical UniversityMoscow

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