Bulletin of Experimental Biology and Medicine

, Volume 127, Issue 1, pp 33–36 | Cite as

Induced cell death and its role in hemopoietic hypoplasia after cytostatic treatment

  • E. D. Gol'dberg
  • G. V. Karpova
  • E. A. Timina
  • I. A. Khlusov
General Pathology and Pathological Physiology


The morphological signs of apoptosis in the bone-marrow cells and thymocytes, indices of cellularity in these organs and in peripheral blood and the absolute number of committed bone marrow cells-precursors have been studied on CBA mice injected with Etoposid (1/2 LD50). The results of the study suggest that reduced cellular counts observed in the hemopoietic organs 3–6 h after the cytostatic injection are due to Etoposid-induced apoptosis.

Key words

Etoposid apoptosis morphology bone marrow thymus peripheral blood 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    L. M. Belen'kii,Quantitative Assessment of Pharmacological Effect [in Russian], Leningrad, 81–106 (1963).Google Scholar
  2. 2.
    E. B. Vladimirovskaya, A. A. Maschan, A. G. Rumyantsev,Gematol. I Transfuziol., N 5, 4–9 (1997).Google Scholar
  3. 3.
    M. V. Golovanov,Gematol. I. Transfuziol., N 7, 39–40 (1991).Google Scholar
  4. 4.
    E.D. Goldberg, A. M. Dygai, and V. P. Shakhov,Tissue-Culture Methods in Hematology [in Russian], Tomsk (1992).Google Scholar
  5. 5.
    G. F. Lakin, Biological measurements, Moscow (1991).Google Scholar
  6. 6.
    V. M. Pogorelov, G. I. Kozinets,Gematol. i. Transfuziol., N 5, 17–25 (1995).Google Scholar
  7. 7.
    S. R. Umanskii,Molekul. Onkol.,30, N 3, 487–501 (1996).Google Scholar
  8. 8.
    P. D. Allen, X. R. Jiang, S. M. Kesley, et al.,British J Haematol.,86, N 1, 50 (1994).Google Scholar
  9. 9.
    B. G. Del, Z. Darzynkiewicz,Cancer Res.,51, N 4, 1165–1169 (1991).Google Scholar
  10. 10.
    J. F. R. Kerr, A. N. Wyllie, and A. R. Currie,Br. J. Cancer,26, N 3, 239–257 (1972).PubMedGoogle Scholar
  11. 11.
    J. F. R. Kerr, C. M. Winterford, and B. V. Harmon,Cancer,73, N 8, 2013–2026 (1994).PubMedCrossRefGoogle Scholar
  12. 12.
    S. J. Martin, D. R. Green, and Th. G. Cotter,Trends Biochem. Sci.,19, 26–30 (1994).PubMedCrossRefGoogle Scholar
  13. 13.
    C. Pellicciari, M. G. Bottone, V. Schaack, et al.,Eur. J. Histochem.,40, N 4, 289–298 (1996).PubMedGoogle Scholar
  14. 14.
    P. R. Walker, C. Smith, T. Youdale, et al.,Cancer Res.,51, N 4, 1078–1085 (1991).PubMedGoogle Scholar
  15. 15.
    H. Yoshidal, M. Takase, T. Bando, et al.,Cancer J.,6, N 4, 220–228 (1993).Google Scholar

Copyright information

© Kluwer Academic/Plenum Publishers 1999

Authors and Affiliations

  • E. D. Gol'dberg
    • 1
  • G. V. Karpova
    • 1
  • E. A. Timina
    • 1
  • I. A. Khlusov
    • 1
  1. 1.Institute of Pharmacology, Tomsk Research CenterRussian Academy of Medical SciencesTomsk

Personalised recommendations