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Evaluation of activity of cytochrome P-450-dependent monooxygenase system induced by β-naphthoflavone and dexamethasone in rats at different ages

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Abstract

This study was undertaken to investigate the effects of age and some inducers (BNF-40 mg/kg/day, DEX − 20 mg/kg/day for 3 days each) on hepatic mixed function oxidase. Wistar male rats of very young (0.5 and 1 months), older (2, 4, 8,12 months) and very old (20 and 28 months) ages were used. The cytochrome P-450 content and the activity of NADH-cytochrome b5 reductase were highest in one-month-old animals, while the cytochrome b5 content was highest in the youngest but NADPH-cytochrome P-450 activity in the oldest rats. β-Naphthoflavone (BNF) treatment resulted in an enhanced content of cytochrome P-450 in all age groups but the activity NADPH-cytochrome P-450 reductase was increased only in the oldest rats. Dexamethasone (DEX) treatment led to a decrease in the activity of NADPH-cytochrome P-450 reductase in the oldest animals only. The cytochrome P-450 content was decreased in young animals (0.5-, 1-, 2-month-old), too. BNF and DEX decreased the cytochrome b 5 content and the activity of NADH-cytochrome b5 in all age groups.

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Authors and Affiliations

  1. II Department of Histology & Embryology, Silesian School of Medicine, PL-40-752, Katowice-Ligota, Poland

    Andrzej Plewka & Danuta Plewka

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  1. Andrzej Plewka
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  2. Danuta Plewka
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Additional information

This work is part five of a series of papers: (1) A. Plewka, M. Bienioszek, Arch. Toxicol., 68:32–36 (1994); (2) A. Plewka, Arch. Gerontol. Ger. 20:149–158 (1995); (3) A. Plewka, M. Bienioszek, D. Plewka, Mech. Ageing Dev., 74:79–88 (1994); (4) A. Plewka, M. Kaminski, D. Plewka, Exp. Gerontol. (submitted).

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Plewka, A., Plewka, D. Evaluation of activity of cytochrome P-450-dependent monooxygenase system induced by β-naphthoflavone and dexamethasone in rats at different ages. AGE 18, 159–165 (1995). https://doi.org/10.1007/BF02432630

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