AGE

, Volume 13, Issue 3, pp 65–71 | Cite as

Nifedipine influences rotifer lifespan: Studies on the calcium theory of aging

  • Alison McTavish
  • Masaaki Sawada
  • Hildegard E. Enesco
Article
  • 38 Downloads

Abstract

The results presented here show that there is an increase in calcium uptake in the cells of the rotifer Asplanchna brightwelli as it ages. The results further show that the 1,4-dihydropyridine calcium channel blocker nifedipine prevents this age-related increase in calcium uptake when administered to rotifers at a concentration of 1.0 μM. A range of nifedipine concentrations from 0.1 μM to 5.0 μM significantly increased rotifer lifespan. Thus, prevention of an age-associated increase in calcium uptake is correlated with increased lifespan in rotifers. These findings suggest that there is an age-related decline in calcium homeostasis, and support the theory that calcium ion regulation is a vital factor in aging rotifer tissues.

Keywords

Calcium Calcium Channel Nifedipine Channel Blocker Calcium Channel Blocker 

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References

  1. 1.
    Michaelis, H.L.: Molecular mechanisms underlying age-dependent alterations in calcium homeostasis: the need for more information and new tools. Neurobiol. of Aging, 8: 348–350, 1987.CrossRefGoogle Scholar
  2. 2.
    Carafoli, E.: Intracellular calcium homeostasis. Ann. Rev. Biochem., 56: 395–433, 1987.PubMedCrossRefGoogle Scholar
  3. 3.
    Campbell, A.K.: Intracellular calcium: its universal role as regulator. John Wiley & Sons Ltd., New York, 1983.Google Scholar
  4. 4.
    Gibson, G.E., and Peterson, C.: Calcium and the aging nervous system. Neurobiol. of Aging, 8: 329–343, 1987.CrossRefGoogle Scholar
  5. 5.
    Farber, J.L.: The role of calcium in cell death. Life Sciences, 29: 1289–1295, 1981.PubMedCrossRefGoogle Scholar
  6. 6.
    Seisjo, B.K.: Cell damage in the brain: a speculative synthesis. J. of Cerebral Blood Flow and Metabol. 1: 155–185, 1981.Google Scholar
  7. 7.
    Khachaturian, Z.S.: Hypothesis on the regulation of cytosol calcium concentration and the aging brain. Neurobiol. of Aging, 8: 345–346, 1987.CrossRefGoogle Scholar
  8. 8.
    Leslie, S.W.: Comments on review by Gibson and Peterson, “Calcium and the aging nervous system.” Neurobiol. of Aging, 8: 351–352, 1987.Google Scholar
  9. 9.
    Trabucchi, M., and Govoni, S.: Drugs affecting calcium movements and the aging brain. Neurobiol. of Aging, 8: 355–357, 1987.CrossRefGoogle Scholar
  10. 10.
    Trump, B.F., Berezesky, I.K., Sato, T., Laiho, K.V., Phelps, P.C., and Declaris, N.: Cell calcium, cell injury and cell death. Environ. Health Perspec., 57: 281–287, 1984.Google Scholar
  11. 11.
    Roth, G.S.: Mechanisms of altered hormone and neurotransmitter action during aging: the role of impaired calcium mobilization. Ann. N.Y. Acad. Sci., 521: 170–176, 1988.PubMedGoogle Scholar
  12. 12.
    Gee, M.V., Ishakawa, Y., Baum, B.J., and Roth, G.S.: Impaired adrenergic stimulation of rat parotid cell glucose oxidation during aging: the role of calcium. J. of Gerontol., 41:331–335, 1986.Google Scholar
  13. 13.
    Hess, P., Lansman, J.B., and Tsien, R.W.: Different modes of calcium channel gating behavior favoured by dihydropyridien calcium agonists and antagonists. Nature, 311: 538–544, 1984.PubMedCrossRefGoogle Scholar
  14. 14.
    Steen, P.A., Newberg, L.A., Milde, J.A., and Michenfelder, J.D.: Cerebral blood flow and neurologic outcome in the dog. J. Cerebral Blood Flow and Metabol., 4: 82–87, 1984.Google Scholar
  15. 15.
    Grotta, J., Spydell, J., Pettigrew, C., Ostrow, P., and Hunter, D.: The effect of nicardipine on neuronal function following ischemia. Stroke, 17: 213–219, 1986.PubMedGoogle Scholar
  16. 16.
    Massie, R., Aiello, V.R., Williams, T.R., and DeWolfe, L.K.: Calcium and calmodulin changes with aging in Drosophila. Age, 12:7–11, 1989.Google Scholar
  17. 17.
    Sincock, A.M.: Life extension in the rotifer Mytilina brevispina var redunca. J. Gerontol. 29: 514–517, 1974.PubMedGoogle Scholar
  18. 18.
    Sincock, A.M.: Life extension in the rotifer Mytilina brevispina var redunca by the application of chelating agents. J. Gerontol. 30: 289–293, 1975.PubMedGoogle Scholar
  19. 19.
    Lansing, A.: Increase of cortical calcium with age in the cells of a rotifer, Euchlanis dilata, a planarian, Phagocata sp, and a toad, Bufo fowleri, as shown by the microincineration technique. Biolog. Bull., 82: 392–400, 1942.Google Scholar
  20. 20.
    Beauvais, J.E., and Enesco, H.E.: Lifespan and age-related changes in activity level of the rotifer Asplanchna brightwelli: influence of curare. Exper. Geront. 20: 359–366, 1985.CrossRefGoogle Scholar
  21. 21.
    Miller, R.J.: How many types of calcium channels exist in neurons? Trends in Neuroscience, 8: 45–47, 1985.CrossRefGoogle Scholar
  22. 22.
    Nogrady, T., and Alai, M.: Cholinergic neurotransmission in rotifers. Hydrobiologia, 104: 149–153, 1983.CrossRefGoogle Scholar
  23. 23.
    Cornillac, A., Wurdak, E., and Clement, P.: Phototaxis in monochromatic light and microspectrophotometry of the cerebral eye of the rotifer Brachionus calcyflorus. Hydrobiologia, 104: 191–196, 1983.CrossRefGoogle Scholar
  24. 24.
    Clement, P., and Wurdak, E.: Photoreceptors and photoreception in rotifers. NATO Summer School, 1984, Lennoxville, Canada.Google Scholar
  25. 25.
    Enesco, H.E., Bozovic, V., and Anderson, P.D.: The relationship between lifespan and reproduction in the rotifer Asplanchna brightwelli. Mech. Ageing and Develop., 48: 281–289, 1989.CrossRefGoogle Scholar
  26. 26.
    Lints, F.A.: The rate of living theory revisited. Gerontol. 35: 36–57, 1989.CrossRefGoogle Scholar
  27. 27.
    Khachaturian, Z.S.: Towards theories of brain ageing, in Handbook of Studies on Psychiatry and Old Age, edited by Kay, D.W. and Barrows, G.D., Amsterdam, Elsevier, 1984, pp. 7–30.Google Scholar
  28. 28.
    Khachaturian, Z.S.: The role of calcium regulation in brain aging: reexamination of a hypothesis. Aging, 1: 17–34, 1989.PubMedGoogle Scholar
  29. 29.
    Sawada, M., and Carlson, J.: Association between lipid peroxidation and life-modifying factors in rotifers. J. Gerontol. 42: 451–456, 1987.PubMedGoogle Scholar
  30. 30.
    Engineer, F., and Sridhar, R.: Inhibition of rat heart and liver microsomal lipid peroxidation by nifedipine. Biochem. Pharmacol., 38: 1279–1285, 1989.PubMedCrossRefGoogle Scholar
  31. 31.
    Ondrias, K., Misik, V., Gergel, D., and Stasko, A.: Lipid peroxidation of phosphotidylcholine liposomes depressed by the calcium channel blockers nifedipine and verpamil and by the antiarrhythmic-antihypoxic drug stobadine. Biochim. Biophys. Acta, 1003: 238–245, 1989.PubMedGoogle Scholar
  32. 32.
    Harman, D.: The free radical theory of aging: nutritional influences. Age, 1:145–152, 1978.CrossRefGoogle Scholar
  33. 33.
    Enesco, H.E., and Verdone-Smith, C.: α-Tocopherol increases lifespan in the rotifer Philodina. Exper. Gerontol., 15: 335–338, 1980.CrossRefGoogle Scholar
  34. 34.
    Sawada, M., and Enesco, H.E.: Vitamin E extends lifespan in the short-lived rotifer Asplanchna brightwelli. Exper. Gerontol., 19: 179–183, 1984.CrossRefGoogle Scholar
  35. 35.
    Bozovic, V., and Enesco, H.E.: Effect of antioxidants on rotifer lifespan and activity. Age, 9: 41–45, 1986.Google Scholar
  36. 36.
    McTavish, A.: Calcium, calcium channel blockers and aging in the rotifer Asplanchna brightwelli, Grosse. M.Sc. dissertation. Concordia University, Montreal, 1989.Google Scholar
  37. 37.
    Bradford, M.M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analyt. Biochem., 72: 248–2654, 1976.PubMedCrossRefGoogle Scholar
  38. 38.
    Sawada, M.: Radioisotope uptake experiments with the rotifer. Internal report. University of Waterloo. Waterloo, Ontario 1988.Google Scholar
  39. 39.
    Zar, J.H.: Biostatistical Analysis. Prentice-Hall Inc., New Jersey, 1984.Google Scholar

Copyright information

© American Aging Association, Inc. 1990

Authors and Affiliations

  • Alison McTavish
    • 1
  • Masaaki Sawada
    • 1
  • Hildegard E. Enesco
    • 1
  1. 1.Department of BiologyConcordia UniversityMontrealCanada

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