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AGE

, Volume 13, Issue 2, pp 27–31 | Cite as

Hepatic myc protooncogene expression is reduced and possibly correlated with body temperature in fasted Peromyscus leucopus mice

  • Kenji D. Nakamura
  • Peter H. Duffy
  • Ming-Hsiung Lu
  • Ronald W. Hart
Article

Abstract

To examine the effect of fasting on c-myc protooncogene expression, the level of c-myc mRNA was determined at 6 circadian stages (4 hr intervals for 24 hr) in the livers of Peromyscus leucopus mice. After 3 days of fasting a reduced level of c-myc expression was observed compared with ad libitum fed controls (24-hr mean relative expression values = 0.73 and 1.16, respectively, p<.001). Core body temperature, which was continuously monitored using a thermister/transmitter system, showed a significantly lower mean value in the fasted animals (33.1°C vs 36.4°C after 3 days, p<.001). In addition, for both ad libitum fed and fasted mice, expression of c-myc and temperature showed significantly different values at various circadian stages indicating that both parameters exhibited biological rhythms. These findings are qualitatively similar to our previous results with long-term dietary restricted animals (60% of ad libitum for over 100 weeks), indicating that manipulation of the dietary regimen can affect the level of protooncogene expression. Furthermore, the results showed a high degree of correlation between c-myc expression and core body temperature in fasted animals in a manner which suggests that alterations in body temperature precede and may signal subsequent changes in c-myc expression.

Keywords

Body Temperature Core Body Temperature Dietary Regimen Biological Rhythm Fast Animal 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© American Aging Association, Inc. 1990

Authors and Affiliations

  • Kenji D. Nakamura
    • 1
  • Peter H. Duffy
    • 1
  • Ming-Hsiung Lu
    • 1
  • Ronald W. Hart
    • 1
  1. 1.DHHSIPHS/Food and Drug AdministrationNational Center for Toxicological ResearchJeffersonUSA

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