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The surface membrane proteins of the WI-38 fibroblast in relation to transformation and aging

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Abstract

The urea extractable cell surface proteins of WI-38 human embryonic fibroblasts and SV-40 transformed WI-38 fibroblasts were examined. Electrophoretic analysis on 5.6% SDS polyacrylamide gels revealed 10–15 discrete protein bands. Three of these bands, of molecular weight 50,000, 45,000 and 30,000 daltons, were found to be present in increased amounts in transformed fibroblasts. The expression of these proteins decreased with increasing culture age. Thus a direct relationship exists between the expression of these proteins and the proliferative state of the culture.

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  1. Worcester Polytechnic Institute, Worcester, Massachusetts, 01609

    Michael E. Palumbo

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  1. Michael E. Palumbo
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Palumbo, M.E. The surface membrane proteins of the WI-38 fibroblast in relation to transformation and aging. AGE 2, 1–4 (1979). https://doi.org/10.1007/BF02432207

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