Biochemical and cytological analyses in natural populations ofVicia benghalensis L.
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27 accessions ofVicia benghalensis from different geographical origins constitute the pool on which the present study was performed. Genetic variation among the samples was biochemically and cytologically evaluated: seed storage protein profiles and C-banded karyotypes were analysed from single individuals of each accession.
SDS-PAGE has shown the possibility to divide the samples into two groups, each characterized by specific protein profiles. The two patterns were indicated as A and B. From individual seed electrophoresis it was ascertained that samples possessing the pattern A showed a low level of individual variation, while those possessing the pattern B were highly polymorphic, thus suggesting differences in the allogamic rate.
The cytological analysis demonstrated the presence of two groups of accessions, one being much richer in heterochromatin as evidenced by C-banding (H+) than the other (H-). The analysis of biochemical and karyological data showed a constant association between pattern A and karyotype H− and between pattern B and karyotype H+.
On the basis of these results it is proposed to considerV. benghalensis as a highly heteromorphic species, in which two groups may be identified.
Key wordsheterochromatin rate of allogamy microevolution seed proteins Vicia
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