Advertisement

The Journal of Membrane Biology

, Volume 4, Issue 1, pp 395–407 | Cite as

Microautoradiographic investigations of sulfate uptake by glands and epidermal cells of water lily (Nymphaea) leaves with special reference to the effect of poly-l-lysine

  • Ulrich Lüttge
  • Charles K. Pallaghy
  • Katharina von Willert
Article

Summary

The uptake of35S-labelled sulfate ions into hydropote cells (densely cytoplasmic gland cells) and into epidermal cells (highly vacuolated cells) ofNymphaea leaves is dependent on metabolic energy. Only a very small fraction of the accumulated35S is incorporated into organic macromolecules during the experimental period. Both cell types exhibit a hyperbolic isotherm for35S uptake from labelled K2SO4 solutions over an external concentration range of 0 to 0.5mm. Although the gland and epidermal cells behave qualitatively similarly, the glands generally absorb about twice as much35S per unit area of sections of the cells as do the epidermal cells. At 3 °C, poly-l-lysine concentrations of 10−8 m and up to 10−7 m enhance35S uptake by the epidermal and gland cells for the first 7.5 hr after application of the poly-l-lysine. Samples treated with 5×10−7 m poly-l-lysine are indistinguishable from the controls over the same period. After longer periods of treatment with poly-l-lysine (7.5 to 24 hr), the rates of35S uptake were reduced by all poly-l-lysine concentrations between the range 10−8 to 5×10−7 m. After 7.5 hr of35S uptake, the control samples contained the smallest amount of label, but after an uptake period of 24 hr the amount of label in the controls is considerably larger than in samples treated with poly-l-lysine. The results suggest that poly-l-lysine increases the membrane permeability and alters the metabolic uptake of sulfate in both hydropotes and epidermal cells.

Keywords

Epidermal Cell Sulfate Uptake Water Lily Blank Slide Anion Pump 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Boyd, G. A. 1955. Autoradiography in Biology and Medicine. Academic Press, New York.Google Scholar
  2. Briggs, G. E. 1963. Rate of uptake of salts by plant cells in relation to an anion pump.J. Exp. Bot. 14:191.Google Scholar
  3. Dilley, R. A. 1968. Effect of poly-l-lysine on energy-linked chloroplast reactions.Biochemistry 7:338.PubMedCrossRefGoogle Scholar
  4. Dowd, J. E., Riggs, D. S. 1965. A comparison of the estimates of Michaelis-Menten kinetic constants from various linear transformations.J. Biol. Chem. 240:863.PubMedGoogle Scholar
  5. Epstein, E. 1966. Dual pattern of ion absorption by plant cells and by plants.Nature 212:1324.CrossRefGoogle Scholar
  6. Johnson, C. L., Mauritzen, C. M., Starbuck, W. C., Schwartz, A. 1967. Histones and mitochondrial ion transport.Biochemistry 6:1121.PubMedCrossRefGoogle Scholar
  7. Kristen, U. 1969. Licht- und elektronenmikroskopische Untersuchungen an den Hydropoten vonNuphar lutea, Nymphoides peltata, Sagittaria macrophylla undSalvinia auriculata.Flora Abt. A. 159:536.Google Scholar
  8. Läuchli, A., Lüttge, U. 1968. Untersuchung der Kinetik der Ionenaufnahme in das Cytoplasma vonMnium-Blattzellen mit Hilfe der Mikroautoradiographie und der Röntgenmikrosonde.Planta 83:80.CrossRefGoogle Scholar
  9. Lüttge, U. 1964. Mikroautoradiographische Untersuchungen über die Funktion der Hydropoten vonNymphaea.Protoplasma 59:157.CrossRefGoogle Scholar
  10. —, Krapf, G. 1969. Die Ultrastruktur derNymphaea-Hydropoten in Zusammenhang mit ihrer Funktion als salztransportierende Drüsen.Cytobiologie 1:121.Google Scholar
  11. —, Weigl, J. 1965. Zur Mikroautoradiographie wasserlöslicher Substanzen.Planta 64:28.CrossRefGoogle Scholar
  12. Osmond, C. B., Laties, G. G. 1970. Effect of poly-l-lysine on potassium fluxes in red beet tissue.J. Membrane Biol. 2:85.CrossRefGoogle Scholar
  13. Reynolds, E. S. 1963. The use of lead citrate at high external pH as an electronopaque stain in electron microscopy.J. Cell Biol. 17:208.PubMedCrossRefGoogle Scholar
  14. Schwartz, A. 1965. The effect of histones and other polycations on cellular energetics. I. Mitochondrial oxidative phosphorylation.J. Biol. Chem. 240:939.PubMedGoogle Scholar
  15. Torii, K., Laties, G. G. 1966. Mechanisms of ion uptake in relation to vacuolation of corn roots.Plant Physiol. 41:863.PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag New York Inc 1971

Authors and Affiliations

  • Ulrich Lüttge
    • 1
    • 2
  • Charles K. Pallaghy
    • 1
    • 2
  • Katharina von Willert
    • 1
    • 2
  1. 1.Botanisches Institut der Technischen HochschuleDarmstadtGermany
  2. 2.Research School of Biological SciencesAustralian National UniversityCanberraAustralia

Personalised recommendations