The mechanism of action of valinomycin on the thylakoid membrane
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Most of the studies devoted to the mechanism by which certain antibiotics increase the ion permeability ofbiological membranes have been carried out on artificialmodel systems. Undoubtedly one of the major reasons for this was that some of the most relevant biological membrane systems are of submicroscopic dimensions and thus inaccessible to the common electrochemical measuring techniques. This holds for the inner membrane systems of chloroplasts, mitochondria, and retinal rods.
Since it is not trivial that a mechanism of action found for a model membrane works as well in a biological one with a much higher structural complexity, it seemed worth-while to study the mechanism of action of ionophorous antibiotics on the above-mentioned biological membranes.
In this paper, a nonelectrochemical method for measuring both the voltage and the current across the inner chloroplast membrane (or thylakoid membrane) is established in extension of earlier work. This method is used to characterize the mode of action of valinomycin on the thylakoid membrane.
KeywordsThylakoid Membrane Absorption Change Valinomycin Electric Potential Difference Intrinsic Conductivity
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- Chappel, I. B., Crofts, A. R. 1966. Ion transport and reversible volume changes of isolated mitochondria.In: The Regulation of Metabolic Processes in Mitochondrion, p. 293. Elsevier Publishing Company, Amsterdam.Google Scholar
- Döring, G., Stiehl, H. H., Witt, H. T. 1967. A second chlorophyll reaction in the electron chain of photosynthesis—Registration by the repetitive excitation technique.z. Naturf. 22b:639.Google Scholar
- —, Emrich, H. M., Witt, H. T. 1970. The indication of a light induced electrical field by pigments incorporated in chloroplast membranes.In: Proc. Coral Gables Conf. on the Physical Princ. of Biological Membranes (Dec. 1968), p. 383. Gordon and Breach Science Publishers, New York.Google Scholar
- —, Witt, H. T. 1968. On the ion transport system of photosynthesis—Investigation on a molecular level.Z. Naturf. 23b:244.Google Scholar
- Kreutz, W. 1970. X-Ray structure research on the photosynthesis membrane.In: Advances in Botanical Research, Vol. III. R. D. Proston, editor. p. 53, Academic Press, New York.Google Scholar
- Lev, A. A., Bujinsky, E. P. 1967. Cation specificity of bimolecular phospholipid membranes containing the valinomycin.Tsitologiya (USSR) 9:102.Google Scholar
- Liberman, Ye. A., Topaly, V. P. 1968. Transfer of ions across bimolecular membranes and classification of uncouplers of oxidative phosphorylation.Biophysics (USSR) Engl. Transl. 13:1195.Google Scholar
- Rüppel, H., Witt, H. T. 1969. Measurements of fast reactions by single and repetitive excitations with pulses of electromagnetic radiation.In: Fast Reactions, Methods in Enzymology, Vol. XI. p. 317. Academic Press, New York.Google Scholar
- Schliephake, W., Junge, W., Witt, H. T. 1968. Correlation between field formation, proton translocation, and the light reactions in photosynthesis.Z. Naturf. 23b:1571.Google Scholar
- Shemyakin, M. M., Ovchinnikov, Yu. A., Ivanov, V. T., Antonov, V. K., Vinogradova, E. I., Shkrob, A. M., Malenkov, G. G., Evstratov, A. V., Laine, I. A., Melnik, E. I., Ryabova, I. D. 1969. Cyclodepsipeptides as chemical tools for studying ionic transport through membranes.J. Membrane Biol. 1:402.CrossRefGoogle Scholar
- Witt, H. T., Rumberg, B., Junge, W. 1968. Electron transfer, field changes, proton translocation and phosphorylation in photosynthesis. Coupling in the thylakoid membrane.In: 19. Mosbach-Colloquium (April 1968). p. 262. Springer Verlag, Berlin.Google Scholar
- Wolff, C., Buchwald, H.-E., Rüppel, H., Witt, K., Witt, H. T. 1969. Rise time of the light induced electrical field across the function membrane of photosynthesis. Registration by repetitive laser giant pulse photometry.Z. Naturf. 24b:1038.Google Scholar