The Journal of Membrane Biology

, Volume 4, Issue 1, pp 42–51 | Cite as

Mechanism of lipid activation of Na, K, Mg-activated adenosine triphosphatase and K, Mg-activated phosphatase of bovine cerebral cortex

  • Ryo Tanaka
  • Terufumi Sakamoto
  • Yumiko Sakamoto
Article

Summary

Na+, K+, Mg++-activated adenosine triphosphatase and K+, Mg++-activatedp-nitrophenyl phosphatase prepared from a membrane fraction of bovine cerebral cortex were studied with regard to the manner of their activation by phospholipids, using phosphatidyl serine, lysolecithin, monodecyl and didecyl phosphates. The kinetic and chromatographic studies suggested the following. (1) When the enzyme proteins bind the phospholipids in a proper ratio, they attain the optimum activation. (2) The binding causes a simple conversion of the enzymes from an inactive form to a fully activated form. (3) The lipids in both micellar form and molecular dispersion activate the enzymes. (4) Of the proteins contained in the enzyme preparation, only a group of proteins possessing the ATPase and the phosphatase activities bind phospholipids, and the amount of the bound lipids is limited.

Keywords

Critical Micelle Concentration Enzyme Preparation Phosphatidyl Serine Enzyme Protein Adenosine Triphosphatase 

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References

  1. Abood, L. G., Koyama, I., Thomas, V. 1964. Relationship of depolarization to phosphorus metabolism and transport in excitable tissues.Amer. J. Physiol. 207:1438.Google Scholar
  2. Brown, D. A., Malkin, T., Maliphant, G. K. 1955. An X-ray examination of long-chain alkyl dihydrogen phosphates and dialkyl hydrogen phosphates and their sodium salts.J. Chem. Soc. p. 1584.Google Scholar
  3. Green, D. E., Tzagoloff, A. 1966. Role of lipids in the structure and function of biological membranes.J. Lipid Res. 7:587.PubMedGoogle Scholar
  4. Layne, E. 1957. Spectrophotometric and turbidimetric methods for measuring proteins.In: Methods in Enzymology, vol. 3. S. P. Colowick and N. O. Kaplan, editors. p. 447. Academic Press, New York.Google Scholar
  5. Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J. 1951. Protein measurement with the Folin phenol reagent.J. Biol. Chem. 193:265.PubMedGoogle Scholar
  6. Martin, R. G., Ames, B. N. 1961. A method for determining the sedimentation behavior of enzymes: Application to protein mixtures.J. Biol. Chem. 236:1372.PubMedGoogle Scholar
  7. Nelson, A. K., Tay, A. D. F. 1963. The preparation of long-chain monoalkyl phosphates from pyrophosphoric acid and alcohols.Inorg. Chem. 2:775.CrossRefGoogle Scholar
  8. Paddy, J. F. 1969. Surface tension. Part II. The measurement of surface tension.In: Surface and Colloid Science, vol. 1. E. Matijevic, editor. p. 101. Wiley Interscience, New York.Google Scholar
  9. Preston, W. C. 1948. Some correlating principles of detergent action.J. Phys. Colloid Chem. 52:84.CrossRefGoogle Scholar
  10. Skidmore, W. D., Entenman, C. 1962. Two-dimensional thin layer chromatography of rat liver phosphatides.J. Lipid Res. 3:471.Google Scholar
  11. Skipski, V. P., Peterson, R. F., Barclay, M. 1964. Quantitative analysis of phospholipids by thin-layer chromatography.Biochem. J. 90:374.PubMedGoogle Scholar
  12. Tanaka, R. 1969. Comparison of lipid effects on K+−Mg2+-activatedp-nitrophenyl phosphatase and Na+−K+−Mg2+-activated adenosine triphosphatase of membrane.J. Neurochem. 16:1301.PubMedGoogle Scholar
  13. —, Mitsumata, T. 1969.p-Nitrophenyl phosphatase of a membrane fraction from bovine cerebral cortex.J. Neurochem. 16:1163.PubMedGoogle Scholar
  14. —, Sakamoto, T. 1969. Molecular structure in phospholipid essential to activate (Na+−K+−Mg2+)-dependent ATPase and (K+−Mg2+)-dependent phosphatase of bovine cerebral cortex.Biochim. Biophys. Acta 193:384.PubMedCrossRefGoogle Scholar
  15. —, Strickland, K. P. 1965. Role of phospholipid in the activation of Na+, K+-activated adenosine triphosphatase of beef brain.Arch. Biochem. Biophys. 111:583.PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag New York Inc 1971

Authors and Affiliations

  • Ryo Tanaka
    • 1
  • Terufumi Sakamoto
    • 1
  • Yumiko Sakamoto
    • 1
  1. 1.Center for Brain ResearchUniversity of RochesterRochester

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