Quantification of viable biomass in biofilm reactors by extractable lipid phosphate
- 67 Downloads
Lipid phosphate, which is a measure of viable biomass, was determined using biofilm samples from three different laboratory-scale reactors. The analysis procedure proposed in the literature was modified and tested for suitability in experiments with biofilm reactors. The microbial contents of the biofilms studied are compared in three types of reactor.
KeywordsDilution Rate Municipal Sewage Sludge Lipid Phosphate Viable Biomass Chloroform Volume
Unable to display preview. Download preview PDF.
- APHA, American Public Health Association (1989) Standard methods for the examination of water and waste water, 17th edn. Norton WW, WashingtonGoogle Scholar
- Bartlett GR (1959) Phosphorus assay in column chromatography. J Biol Chem 234:466–468Google Scholar
- Bligh EG and Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37:911–918Google Scholar
- Cao YS, Alaerts GJ, Hooijmans CM, Lubberding HJ (1992) Oxygen consumption and organics decomposition in drainage systems with attached biofilm. Water Sci Technol 26:683–692Google Scholar
- Derikx PJL, Op den Kamp HJM, Drift G van de, Van Griensven LJLD, Vogels GD (1990) Biomass and biological activity during the production of compost used as a substrate in mushroom cultivation. Appl Environ Microbiol 56:3029–3034Google Scholar
- Federle TW, White DC (1982) Preservation of estuarine sediments for lipid analysis of biomass and community structure of microbiota. Appl Environ Microbiol 44:1166–1169Google Scholar
- Findlay RH, King GM, Watling L (1989) Efficiency of phospholipid analysis in determining microbial biomass in sediments. Appl Environ Microb 55:2888–2893Google Scholar
- Henson JM, Smith PH, White DC (1985) Examination of thermophilic methane-producing digestors by analysis of bacterial lipids. Appl Environ Microbiol 50:1428–1433Google Scholar
- McKinley VL, Vestal JR (1985) Physical and chemical correlates of microbial activity and biomass in composting municipal sewage sludge. Appl Environ Microbiol 50:1395–1403Google Scholar
- Singer SJ, Nicolson GL (1972) The fluid mosaic model of the structure of membranes. Science 175:720–731Google Scholar
- Wilderer PA, Characklis WG (1989) Structure and function of biofilms. Life Sci Res Rep 46:5–18Google Scholar