AGE

, Volume 6, Issue 2, pp 52–56 | Cite as

Absorption of polyethylene glycol 400 administered orally to mature and senescent rats

  • Chu-Fang Lin
  • William L. Hayton
Article

Abstract

Absorption of polyethylene glycol 400 administered orally to mature and senescent rats. Lin, C.F., and Hayton, W.L. Polyethylene glycol 400 (PEG 400), a probe for studying GI absorptive capacity, is a mixture of homologous compounds differing in molecular weight (mw). The bio-availability of PEG 400 was studied in senescent (age 32–37 months) and mature (age 17–20 months) male Sprague-Dawley rats, by giving PEG 400 both orally and intravenously and measuring the amounts of the component molecular species excreted into the urine. After intravenous administration, urinary recovery of the various components increased with an increase in mw. The pattern of urinary recovery was similar in both groups, and led to the conclusion that the urinary disposition of PEG 400 was not affected by senescence. After oral administration, the extent of absorption of the low mw components was higher but that of the high mw components was lower in senescent rats than in the mature animals. These senescence-related alterations in the bioavailability of PEG 400 were attributed to age-dependent changes in intestinal transit rate and mucosal permeability. Similar results were observed in a preliminary longitudinal study.

Keywords

Molecular Species Polyethylene Glycol Absorptive Capacity Intestinal Transit Mature Animal 

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References

  1. 1.
    Chadwick, V. S., Phillips, S. F., and Hofmann, A. F.: Measurements of intestinal permeability using low molecular weight polyethylene glycols (PEG 400). II. Application to normal and abnormal permeability states in man and animals. Gastroenterol., 73:247–251, 1977.Google Scholar
  2. 2.
    Chadwick, V. S., Phillips, S. F., and Hofmann, A. F.: Measurements of intestinal permeability using low molecular weight polyethylene glycols (PEG 400). I. Chemical analysis and biological properties of PEG 400. Gastroenterol., 73:241–246, 1977.Google Scholar
  3. 3.
    Tagesson, C., Sjodahe, R., and Thoren, B.: Passage of molecules through the wall of the gastrointestinal tract. I. A simple experimental model. Scand. J. Gastroenterol., 13:519–524, 1978.PubMedCrossRefGoogle Scholar
  4. 4.
    Sundqvist, T., Magnusson, K-E., Sjodahl, R., Stjernstrom, I., and Tagesson, C.: Passage of molecules through the wall of the gastrointestinal tract. II. Application of low-molecular weight polyethylene glycol and a deterministic mathematical model for determining intestinal permeability in man. Gut, 21:208–214, 1980.PubMedGoogle Scholar
  5. 5.
    Lin, C. F., and Hayton, W. L.: GI motility and subepithelial blood flow in mature and senescent rats. Age 6:46–51, 1983.Google Scholar
  6. 6.
    Hollander, D., and Morgan, D.: Increase in cholesterol intestinal absorption with aging in the rat, Exp. Geront., 14:201–204, 1979.CrossRefGoogle Scholar
  7. 7.
    Hollander, D., and Morgan, D.: Aging: its influence on vitamin A intestinal absorption in vivo by the rat. Exp. Geront., 14:301–305, 1979.CrossRefGoogle Scholar
  8. 8.
    Salem, H., Grossman, M. H., and Bilbey, D. L.: Micro-method for intravenous injection and blood sampling. J. Pharm. Sci., 52:794–795, 1963.PubMedGoogle Scholar
  9. 9.
    Nightingale, C. H., and Mourarieff, M.: Reliable and simple method of intravenous injection into the laboratory rat. J. Pharm. Sci., 62:860–861, 1973.PubMedGoogle Scholar
  10. 10.
    Steel, R. G. D., and Torrie, J. H.: Analysis of variance III: factorial experiments, in Principles and Procedures of Statistics: a Biometrical Approach, 2nd edition, edited by Napier, C., and Maisel, J. W., N.Y., McGraw-Hill Book Co., 1980, pp. 377–398.Google Scholar

Copyright information

© American Aging Association, Inc. 1983

Authors and Affiliations

  • Chu-Fang Lin
    • 1
  • William L. Hayton
    • 1
  1. 1.College of PharmacyWashington State UniversityPullman

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