AGE

, Volume 7, Issue 2, pp 36–41 | Cite as

Lipofuscin in mouse placenta: Variations with age

  • Anita M. Brown
  • Hildegard E. Enesco
Article
  • 46 Downloads

Abstract

The question under study here is whether the placenta may serve as a model for the study of cell or tissue aging. Since lipofuscin accumulation is generally recognized as an age-related phenomenon, we set out to determine whether lipofuscin could be detected in mouse placenta, and to determine whether its level changed during the development or aging of the placenta. Lipid peroxidation fluorescence products, believed to represent lipofuscin, were extracted from placentas on days 12, 15, 17 and 19 during the 21-day gestation period and measured spectrofluorometrically. The placenta attained its full weight by the fifteenth day. The level of lipid peroxidation products was highest on day 12, lowest on day 15, and then increased gradually to day 19. Measurements of placental malondialdehyde, a lipid peroxidation product, followed a similar pattern. Histological analysis showed that there was a 27% increase in the number of autofluorescent lipofuscin granules between day 15 and 19 of gestation. To interpret these results, we must search for an explanation of why the lipid peroxidation products are at their highest levels in the youngest placentas. The explanation may be due to the fact that mechanisms which protect against lipid peroxidation have not yet developed to their full extent in the youngest placentas examined. At 12 days the mouse placenta is still undergoing rapid growth. The period after 15 days when the placenta has attained its full size is characterized by a gradual increase in lipofuscin level. Since the level of lipid peroxidation changes as the placenta develops, we do not have clear evidence of placental aging based on this criteria alone.

Keywords

Lipid Peroxidation Histological Analysis Malondialdehyde Full Extent Lipid Peroxidation Product 

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References

  1. 1.
    Fox, H. and Faulk, W.P.: The placenta as an experimental model. Clinics Endocrinol. Met., 10:57–72, 1981.Google Scholar
  2. 2.
    Fox, H.: The placenta as a model for organ ageing, in Placenta: A neglected experimental animal, edited by Beaconsfield, P. and Vilee, C., New York, Pergamon Press, 1979, pp. 351–78.Google Scholar
  3. 3.
    Rosso, P.: Placenta as an aging organ. Current Concepts Nutrition 4:23–41, 1976.Google Scholar
  4. 4.
    Reichel, W.: Lipofuscin pigment accumulation and distribution in five rat organs as a function of age. J. Gerontol., 23: 145–52, 1968.PubMedGoogle Scholar
  5. 5.
    Strehler, B.L., Mark, D.D., Mildvan, A.S., and Gee, M.V.: Rate and magnitude of age pigment accumulation in the human myocardium. J. Gerontol., 14:430–39, 1959.PubMedGoogle Scholar
  6. 6.
    Tappel, A., Fletcher, B., and Deamer, D.: Effects of antioxidants and nutrients on lipid peroxidation fluorescent products and aging in the mouse. J. Gerontol., 28:415–24, 1973.PubMedGoogle Scholar
  7. 7.
    Brunk, U.T. and Collins, V.P.: Lysosomes and age pigments in cultured cells, in Age Pigments, ed. by Sohal, R.S., Amsterdam, Elsevier/North Holland, 1981, pp. 243–64.Google Scholar
  8. 8.
    Fletcher, B.L., Dillard, C.J., and Tappel, A.L.: Measurement of fluorescent lipid peroxidation products in biological systems and tissues. Analyt. Biochem., 52:1–9, 1973.PubMedCrossRefGoogle Scholar
  9. 9.
    Barber, A.A. and Wilbur, K.M.: The effect of X-irradiation on the antioxidant activity of mammalian tissues. Radiation Research, 10: 167–75, 1959.PubMedGoogle Scholar
  10. 10.
    Barber, A.A. and Bernheim, F.: Lipid peroxidation: Its measurement, occurrence and significance in animal tissues, in Advances in Gerontological Research, edited by Strehler, B.L. New York, Academic Press, 2: 355–403, 1967.Google Scholar
  11. 11.
    Donato, H.: Lipid peroxidation, cross-linking reactions and aging, in Age Pigments, ed. by Sohal, R.S., Amsterdam, Elsevier/North Holland, 1981, pp. 63–81.Google Scholar
  12. 12.
    Elleder, M.: Chemical characterization of age pigments, in Age Pigments, ed. by Sohal, R.S., Amsterdam, Elsevier/North Holland, 1981, pp. 201–41.Google Scholar
  13. 13.
    Brizzee, K.R. and Ordy, J.M. Cellular features, regional accumulation and prospects of modification of age pigments in mammals, in Age Pigments, edited by Sohal, R.S., Amsterdam, Elsevier/North Holland, 1981, pp. 101–54.Google Scholar
  14. 14.
    Munnell, J.F. and Getty, R.: Rate of accumulation of cardiac lipofuscin in the aging canine. J. Gerontol., 23:154–58, 1968.PubMedGoogle Scholar
  15. 15.
    Davies, I. and Fotheringham, A.P.: Lipofuscin — does it affect cellular performance? Exptl. Gerontol., 16:119–25, 1981.CrossRefGoogle Scholar
  16. 16.
    Sohal, R.S. Metabolic rate, aging and lipofuscin accumulation, in Age Pigments, ed. by Sohal, RS. Amsterdam, Elsevier/North Holland, 1981, pp. 306–16.Google Scholar
  17. 17.
    Friede, R.L.: Topographic Brain Chemistry, New York, Academic Press, 1966, pp. 425–30.Google Scholar
  18. 18.
    Friede, R.L.: The relation of the formation of lipofuscin to the distribution of oxidative enzymes in the human brain. Acta Neuropath., 2:113–25, 1962.CrossRefGoogle Scholar
  19. 19.
    Sulkin, N.M. and Srivanij, P.: The experimental production of senile pigments in the nerve cells of young rats. J. Gerontol., 15:2–9, 1960.PubMedGoogle Scholar
  20. 20.
    Kruk, P. and Enesco, H.E.: α-Tocopherol reduces fluorescent age pigment levels in heart and brain of young mice. Experientia, 37:1301–02, 1981.PubMedCrossRefGoogle Scholar
  21. 21.
    Blackett, A.D. and Hall, D.A.: Tissue vitamin E level and lipofuscin accumulation with age in the mouse. J. Gerontol., 36:529–33, 1981.PubMedGoogle Scholar
  22. 22.
    Zuckerman, B.M. and Geist, M.A.: Effect of nutrition and chemical agents on lipofuscin formation, in Age Pigments, ed. by Sohal, R.S., Amsterdam, Elsevier/North Holland, 1981, pp. 283–302.Google Scholar
  23. 23.
    Miquel, J., Lundgren, P.R., and Johnson, J.E.: Spectrophotofluorometric and electron microscopic study of lipofuscin accumulation in the testis of aging mice. J. Gerontol., 33:5–19, 1978.Google Scholar
  24. 24.
    McArthur, M.C. and Sohal, R.S.: Relationship between metabolic rate, aging, lipid peroxidation and fluorescent age pigment in milkweed bug (Oncopeltus fasciatus (Hemiptera). J. Gerontol., 37:268–74, 1982.PubMedGoogle Scholar
  25. 25.
    Parmley, T.H., Gupta, P.K., and Walker, M.A.: “Aging” pigments in term human placenta. Am. J. Obstet. Gynecol., 139:760–66, 1981.PubMedGoogle Scholar
  26. 26.
    Sekiba, K. and Yoshioka, T.: Changes of lipid peroxidation and superoxide dismutase activity in human placenta. Amer. J. Obstet. Gynecol., 135:368–71, 1979.Google Scholar
  27. 27.
    Yoshioka, T., Kawada, K., Shimada, T., and Mori, M.: Lipid peroxidation in maternal and cord blood and protective mechanism against activated-oxygen toxicity in the blood. Amer. J. Obstet. Gynecol., 135:372–76, 1979.Google Scholar
  28. 28.
    Andrew, W.: Anatomy of aging in man and animals, New York, Grume and Stratton, 1971.Google Scholar
  29. 29.
    Enesco, H.E. and Mahoney, D.: Age-related decrease in nuclear and nucleolar size in hypodermal cells of the rotifer. Exptl. Gerontol., 16:41–45, 1981.CrossRefGoogle Scholar
  30. 30.
    Enesco, H.E. and Samborsky, J.: Liver polyploidy: Influence of age and dietary restriction. Exptl. Gerontol., 18:78–87, 1983.Google Scholar
  31. 31.
    Horn, P.L., Laver, J.J., and Wood, J.T.: Changes of aging parameters among rats on diets differing in fat quantity and quality. J. Gerontol., 36:285–93, 1981.PubMedGoogle Scholar
  32. 32.
    Chalkley, H.W.: Method for the quantitative morphologic analysis of tissues. J. Nat. Cancer Inst., 4: 47–53, 1943.Google Scholar
  33. 33.
    Newmark, H.L., Pool, W., Baurenfeind, J.C., and Deritter, E.: Biopharmaceutic factors in parenteral administration of Vitamin E. Pharmacological Science, 64:655–57, 1975.Google Scholar
  34. 34.
    Sokahl, R.R. and Rohlf, F.J.: Biometry (2nd ed). San Francisco, W.H. Freeman and Co., 1981.Google Scholar

Copyright information

© American Aging Association, Inc. 1984

Authors and Affiliations

  • Anita M. Brown
    • 1
  • Hildegard E. Enesco
    • 1
  1. 1.Department of BiologyConcordia UniversityMontreal

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