Cellular and molecular aspects of thymus dependent antibody production in aged C3H/HeBr mice
- 16 Downloads
An age related decline in anti-sheep erythrocyte antibody produced by lymphoid cells in splenic tissue of male C3H/HeBr mice was observed for both IgM and IgG production. The total number of lymphocytes per gram of splenic tissue was diminished, but there was no observed decrease in the spleen size with age. Analyses for enhanced suppressor cell activity as is found in tumor bearing mice of this strain revealed no enhanced suppressor activity in non-tumor bearing aged mice. Analyses of total cellular RNA from old and young mouse splenic lymphocytes revealed 8.5% less total RNA in aged mouse cells. Of the total RNA content, the percent of functional messenger RNA (poly(A)-RNA) declined 19.3% in the aged mouse cells. Isolation of the poly(A) tailed messenger RNA followed by cell-free translation of the message using a reticulocyte lysate system demonstrated that the poly(A)-RNA from young and aged mouse splenic lyphocytes directed protein synthesis to the same extent, indicating that although there was less poly(A)-RNA in the aged mouse cells, the activity of the poly(A)-RNA was relatively equivalent to that of young mouse cells. Translated proteins from poly(A)-RNA directed synthesis analyzed by SDS-polyacrylamide gel electrophoresis suggested a significant decline in immunoglobulin light and heavy chain message in the aged mouse lymphocytes of B cell enriched as well as unfractionated populations. This study suggested that age related declining humoral immunity in C3H/HeBr mice may have resulted from a defect in the B cell or helper T cell compartments, but was not the result of enhanced suppressor activity. This finding was substantiated by an observed decrease in poly(A)-RNA levels in aged mouse lymphoid cells as well as a suggested loss of intrinsic message for antibody synthesis.
KeywordsAged Mouse Young Mouse Spleen Size Splenic Lymphocyte Splenic Tissue
Unable to display preview. Download preview PDF.
- 4.Svedersky, L. P., Dodd, M. C., and Minton, J. P.: Immune suppression by cultured lymphocyte supernates of tumor bearing hosts. J. Surg. Oncology, 12:343–347, 1979.Google Scholar
- 13.Prashad, N., and Cutler, R. G.: Quantitative studies of satellite DNA in different tissues of mice as a function of age. Gerontologist, 12:26–32, 1972.Google Scholar
- 16.MacKinnon, P. C., Simpson, R. A., and MacLennan, C.: in vivo and in vitro techniques used in the study of RNA synthesis in the brains of rats and mice at various ages from birth to senility. J. Anatomy, 104: 351–360, 1969.Google Scholar
- 20.Russell, E. S.: Lifespan and aging patterns, in Biology of the Laboratory Mouse, edited by Green, E. L., New York, McGraw-Hill Press, 1966, pp. 511–519.Google Scholar
- 21.Jerne, N. K., Nordin, A. A., and Henry, C.: The agar plaque technique for recognizing antibody-producing cells, in Cell Bound Antibodies, edited by Amos, B., and Korprowski, H., Philadelphia, Wistar Institute Press, 1963, pp. 109–119.Google Scholar
- 24.Mack, B., and Vassalli, P.: Template activity of RNA from antibody producing tissues. Science, 150: 622–626, 1965.Google Scholar