, Volume 11, Issue 1, pp 7–9 | Cite as

The effect of dietary cellulose on life span and biochemical variables of male mice

  • Gertrude C. Kokkonen
  • Charles H. Barrows


Sixteen month old C57BL/6J male mice were fed either a nutritionally adequate diet or this diet diluted with either 33% or 50% cellulose. The life span of the animals fed the latter two diets was longer than that of the controls, but only statistically significant in the animals fed the diet diluted with 50% cellulose. The rates of cellular protein synthesis were estimated in liver by determining: 1) the half lives of proteins by the rate of disappearance of radioactivity following the intraperitoneal injection of C14 labeled sodium bicarbonate, and 2) the cellular protein levels estimated by the concentration of proteins and DNA of the tissue. The rates of disappearance of radioactivity from the liver proteins of mice were not statistically significantly affected by these dietary regimens. However, there was a 27% decrease in the cellular protein synthesis of liver in the animals fed the diets containing cellulose.


Sodium Cellulose Protein Level Bicarbonate Life Span 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Beauchene, R.E., Bales, C.W., Smith, C.A., Tucker, S.M., and Mason, R.L.: The effect of feed restriction on body composition and longevity of rats. Physiologist, 22: (4), 8, 1979 (abst.)Google Scholar
  2. 2.
    Tucker, S.M., Mason, R.L., and Beauchene, R.E.: Influence of diet and feed restriction on kidney function of aging male rats. J. Gerontol., 31: 264–270, 1976.PubMedGoogle Scholar
  3. 3.
    Weindruch, R. and Walford, R.L.: Dietary restriction in mice beginning at 1 year of age: Effect on life span and spontaneous cancer incidence. Science, 215: 1415–1418, 1982.PubMedGoogle Scholar
  4. 4.
    Barrows, C.H. and Kokkonen, G.C.: The effect of age and diet on biochemical characteristics of the tissues of mice. Age, 8: 102–112, 1985.Google Scholar
  5. 5.
    Barrows, C.H. and Kokkonen, G.C.: The effect of age and diet on the cellular protein synthesis of liver of male mice. Age, 10: 54–57, 1987.Google Scholar
  6. 6.
    Lewis, S.E.M., Goldspink, D.F., Phillips, J.G., Merry, B.J., and Holeham, A.M.: The effects of aging and chronic dietary restriction on whole body growth and protein turnover in the rat. Exp. Gerontol., 20: 253–263, 1985.PubMedCrossRefGoogle Scholar
  7. 7.
    Barrows, C.H.: Nutrition, aging, and genetic program. Am. J. Clin. Nutr., 25: 829–833, 1972.PubMedGoogle Scholar
  8. 8.
    Scornik, D.A.: In vivo rate of translation by ribosomes of normal and regenerating liver. J. Biol. Chem., 249: 3876–3883, 1974.PubMedGoogle Scholar
  9. 9.
    Munro, H.N. and Fleck, A.: The determination of nucleic acids, in Methods of Biochemical Analysis, Vol. 14, edited by Glick, D., New York, Interscience, 1966, pp. 113–176.Google Scholar
  10. 10.
    Ceriotti, G.J.: A microchemical determination of desoxyribonucleic acid. J. Biol. Chem., 198: 297–303, 1952.PubMedGoogle Scholar
  11. 11.
    Miller, G.L.: Protein determination for large numbers of samples. Anal. Chem., 31: 964, 1959.CrossRefGoogle Scholar
  12. 12.
    King, T.E.: Reconstitution of respiratory chain enzyme systems. XII. Some observations on the reconstitutes of succinate oxidase systems from heart muscle. J. Biol. Chem., 283: 4037–4051, 1963.Google Scholar

Copyright information

© American Aging Association, Inc. 1988

Authors and Affiliations

  • Gertrude C. Kokkonen
    • 1
  • Charles H. Barrows
    • 1
  1. 1.Gerontology Research CenterNational Institute on Aging, National Institutes of HealthBaltimore

Personalised recommendations