Fabrication of enzyme-containing cellulose fibre materials from graft copolymers containing sulfo groups

Abstract

An analysis of the experimental data and their comparison with previously published [3] results led to the following conclusions: the rate of interpolymer reactions (IPR) involving lysozyme (LYS) and cellulose graft copolymer containing strongly acid groups (sulfo groups) is much lower than the rate of the reaction between LYS and a weak polyanion grafted to cellulose (graft copolymer of cellulose and polyacrylic acid (PAA) sodium salt); the relative activity (RA) of immobilized LYS decreases with an increase in the duration of the reaction, which could be due to the formation of additional bonds in polyelectrolyte complexes (PEC); with all other conditions being equal, in the case of addition to C-gr-PSSA, the RA of immobilized LYS is lower than in addition to C-gr-PAA; this confirms the opinion advanced above that in the reaction with graft chains of PSSA, the conformation of the macromolecules of the enzyme is twisted to a greater degree than in addition to PAA.