A simple image analysis program for quantitative dot assays
Dot assays are versatile, and are widely used for determining antigens and proteins. Because they require expensive equipment to be quantitative, often only qualitative dot results are reported. However, because the dot pattern is so regular, a simple image analysis program can determine mean dot grey levels, while handling irregular dot outlines, radial variations in colour within a dot, and varying or noisy sheet backgrounds. We describe a dot analysis program that runs on PCs under Windows, and permits quantitative dot assays to be run with inexpensive grayscale scanner input. The program is available from us as source and executable files. We present demonstration results for antigen and protein determinations.
KeywordsGrey Level Ergosterol Fungal Biomass Executable File Average Grey Level
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- Abraham, L.D., Roth, A., Saddler, J.N., and Breuil, C. (1993).Can. J. Bot. 71, 1224–1230.Google Scholar
- Banerjee S., Little J., Chan M., Luck B.T., Breuil C. and Brown D.L. (1994). Can. J. Microbiol. (in press).Google Scholar
- Harlow E. and Lane D., Eds. (1988). Antibodies, a laboratory manual, 726 pp.Google Scholar
- Levin, A.E., and Branton, D. (1986).J. Cell Biol. 103, 518a (abstract).Google Scholar
- Mitchell, L.A. (1988).Plant Disease 72, 664–667.Google Scholar