Biotechnology Techniques

, Volume 8, Issue 5, pp 357–362 | Cite as

A simple image analysis program for quantitative dot assays

  • C. Zagodnik
  • J. A. Olson
  • C. Breuil
  • A. G. Robertson


Dot assays are versatile, and are widely used for determining antigens and proteins. Because they require expensive equipment to be quantitative, often only qualitative dot results are reported. However, because the dot pattern is so regular, a simple image analysis program can determine mean dot grey levels, while handling irregular dot outlines, radial variations in colour within a dot, and varying or noisy sheet backgrounds. We describe a dot analysis program that runs on PCs under Windows, and permits quantitative dot assays to be run with inexpensive grayscale scanner input. The program is available from us as source and executable files. We present demonstration results for antigen and protein determinations.


Grey Level Ergosterol Fungal Biomass Executable File Average Grey Level 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Kluwer Academic Publishers 1994

Authors and Affiliations

  • C. Zagodnik
    • 1
  • J. A. Olson
    • 1
    • 2
  • C. Breuil
    • 1
  • A. G. Robertson
    • 1
    • 3
  1. 1.Forest Products Biotechnology, Department of Wood Science, Faculty of ForestryUniversity of British ColumbiaVancouverCanada
  2. 2.Pulp and Paper Centre, Department Of Chemical EngineeringUniversity of British ColumbiaVancouverCanada
  3. 3.Paprican, Vancouver LaboratoryVancouverCanada

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