Summary
The principle of metal chelate-protein interaction was applied for protein extraction by means of reverse micellar phases. For this purpose, an affinity surfactant was synthesized exposing iminodiacetic acid as the hydrophilic moiety. The application of this substance as a cosurfactant leads to enhanced extraction of proteins exhibiting histidine groups on their surface.
Similar content being viewed by others
References
Coughlin, R.W. and Baclaski, J.B. (1990)Biotech. Progr. 6, 307–309
Flaschel, E. and Friehs, K. (1993)Biotech. Adv. 11, 31–78
Hochuli, E., Bannwarth, W., Döbeli, H., Gentz, R., and Stüber, D. (1988)Bio/Technology, 1321–1325
Johansson, G. (1989) in:Protein Purification: Principles, High Resolution Methods, and Applications, Janson, J.-C. and Rydén, L. (eds.), pp. 330–344, Weinheim: VCH Publishers
Jones, M.M., Pratt, T.H., Mitchell, W.G., Harbison, R.D., and MacDonald, J.S. (1976)J. Inorg. Nucl. Chem. 38, 613–616
Kelley, B.D., Wang, D.I.C., and Hatton, T.A. (1993a)Biotechnol. Bioeng. 42, 1199–1208
Kelley, B.D., Wang, D.I.C., and Hatton, T.A. (1993b)Biotechnol. Bioeng. 42, 1209–1217
Porath, J., Carlsson, J., Olsson, I., and Belfrage, G. (1975)Nature 258, 598–599
Schustolla, D. and Hustedt, H. (1990)DECHEMA Biotech. Conf. 4, 1137–1141
Smith, M.C., Cook, J.A., Furman, T.C., Gesellchen, P.D., Smith, D.P., and Hsiung, H. (1990)ACS Symp. Ser. 427, 168–180
Suh, S.-S. and Arnold, F.H. (1990)Biotechnol. Bioeng. 35, 682–690
van Dam, M.E., Wuenschell, G.E., and Arnold, F.H. (1989)Biotech. Appl. Biochem. 11, 492–502
Woll, J.M., Hatton, T.A., and Yarmush, M.L. (1989)Biotech. Progr. 5, 57–62
Wuenschell, G.E., Naranjo, E., and Arnold, F.H. (1990)Bioproc. Eng. 5, 199–202
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Poppenborg, L., Flaschel, E. Affinity extraction of proteins by means of reverse micellar phases containing a metal-chelating surfactant. Biotechnol Tech 8, 307–312 (1994). https://doi.org/10.1007/BF02428972
Issue Date:
DOI: https://doi.org/10.1007/BF02428972