Biotechnology Techniques

, Volume 8, Issue 5, pp 301–306 | Cite as

Immobilization ofColeus blumei cells in a column reactor using a spray feeding system

  • Blanca C. Martinez
  • Chang-Ho Park


Coleus blumei cells were immobilized in a column reactor packed withLuffa cylindrica pieces. Medium was fed from the top of the column using a spray system and cells maintained high viability for 52 days. Cell growth was slower but rosmarinic acid production was better compared to immobilized cells in the shake flasks.


Rosmarinic Acid Column Reactor Fresh Cell Weight Immobilize Cell Culture Rosmarinic Acid Production 
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Copyright information

© Kluwer Academic Publishers 1994

Authors and Affiliations

  • Blanca C. Martinez
    • 1
  • Chang-Ho Park
    • 1
  1. 1.Department of Agricultural EngineeringUniversity of MinnesotaSt. PaulUSA

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