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Sites of uptake of3H-5-hydroxytryptamine in rat isolated lung

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Summary

The localisation of radioactivity in rat lungs after perfusion of3H-5-hydroxytryptamine (5-HT) was studied by autoradiography. Rat isolated lungs, perfused with Krebs bicarbonate solution, via the pulmonary circulation, were rapidly frozen after the infusion of3H-5-HT. All subsequent handling was carried out at −30 to −40°C. Developed sections were examined by the light microscope and showed that radioactivity was concentrated around the alveoli with little or no label in other parts of the lung. Lungs perfused with3H-5-HT in the presence of mebanazine, a monoamine oxidase inhibitor, showed label in the endothelial cells of arteries and arterioles as well as the alveolar label. Lungs treated with amitriptyline were essentially devoid of label. These results indicate that the site of the avid uptake and metabolism of 5-HT in the lung is the endothelial cells of the vasculature.

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Authors and Affiliations

  1. Smith, Kline and French Laboratories, The Research Institute, Welwyn Garden City, Herts., U.K.

    Shirley A. M. Cross

  2. Department of Pharmacology, Institute of Basic Medical Sciences, Royal College of Surgeons of England, Lincoln’s Inn Fields, London, U.K.

    Valerie A. Alabaster, Y. S. Bakhle & J. R. Vane

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  1. Shirley A. M. Cross
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  2. Valerie A. Alabaster
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  3. Y. S. Bakhle
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  4. J. R. Vane
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Cross, S.A.M., Alabaster, V.A., Bakhle, Y.S. et al. Sites of uptake of3H-5-hydroxytryptamine in rat isolated lung. Histochemistry 39, 83–91 (1974). https://doi.org/10.1007/BF02428554

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  • DOI: https://doi.org/10.1007/BF02428554

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