Summary
Enzyme synthesisin vitro directed by T7 heteroduplex DNA is observed when the H strand [the strand assuming the higher density in combination with poly(G,U)], carries the wild allele. This is true for enzyme synthesis mediated byE. coli RNA polymerase or by T7 phage RNA polymerase.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bøvre, K., Szybalski, W.: Patterns of convergent and overlapping transcription within the b2 region of coliphage λ. Virology38, 614–626 (1969).
Cohen, S. N., Hurwitz, J.: Genetic transcription in bacteriophage λ: Studies of λ mRNA synthesis in vivo. J. molec. Biol.37, 387–406 (1968).
Fuchs, E., Millette, R. L., Zillig, W., Walter, G.: Influence of salts on RNA synthesis by DNA-dependent RNA polymerase fromEscherichia coli. Europ. J. Biochem.3, 183–193 (1967).
Gold, L. M., Schweiger, M.: Synthesis of bacteriophage-specific enzymes directed by DNAin vitro. Meth. in Enzymology20, 539–544 (1971).
Hayashi, M., Hayashi, M. N., Spiegelman, S.: Restriction ofin vivo genetic transcription to one of the complementary strands of DNA. Proc. nat. Acad. Sci. (Wash.)50, 664–672 (1963).
Riley, P. A.: A suggested mechanism for DNA transcription. Nature (Lond.)228, 522–525 (1970).
Scherzinger, E., Herrlich, P., Schweiger, M.: Transcription of T3 and T7 early genes by T3 and T7 RNA polymerases. Molec. gen. Genet.,118, 67–77 (1972).
Summers, W. C., Szybalski, W.: Totally asymmetric transcription of coliphage T7in vivo: Correlation with poly G binding sites. Virology34, 9–16 (1968).
Szybalski, W., Kubinski, H., Hradecna, Z., Summers, W. C.: Analytical and preparative separation of the complementary DNA strands. Meth. in Enzymology21, 383–413 (1971).
Taylor, K., Hradecna, Z., Szybalski, W.: Asymmetric distribution of the transcribing regions on the complementary strands of coliphage λ DNA. Proc. natl. Acad. Sci. (Wash.)57, 1618–1625 (1967).
Zillig, W., Zechel, K., Rabussay, D., Schachner, M., Sethi, V. S., Palm, P., Heil, A., Seifert, W.: On the role of different subunits of DNA-dependent RNA polymerase fromE. coli in the transcription process. Cold. Spr. Harb. Symp. quant. Biol.35, 47–58 (1970).
Zubay, G., Chambers, D. A., Cheong, L. C.: Cell-free studies on the regulation of thelac operon, “The lactose operon”, J. R. Beckwith and D. Zipser eds. Cold Spring Harbor Laboratory, 1970, p. 375–391.
Chamberlin, M., McGrath, J., Waskell, L.: New RNA polymerase fromE. coli infected with bacteriophage T7. Nature (Lond.)228, 227 (1970).
Dunn, J. J., Bautz, F. A., Bautz, E. K. F.: Different template specificities of phage T3 and T7 RNA polymerases. Nature (Lond.) New Biol.230, 94 (1971).
Gold, L. M., Schweiger, M.: Synthesis of bacteriophage-specific enzymes directed by DNAin vitro. Methods in Enzymology20, 537 (1971).
Hausmann, R., Härle, E.: Expression of the genomes of the related bacteriophages T3 and T7. Proc. 1st Eur. Biophysics Congr. Baden 1971, Wiener Med. Akademie, vol. I, p. 467 (1971).
Herrlich, P., Scherzinger, E., Schweiger, M.: Differential influence of spermidine on DNA directed enzyme synthesisin vitro. Molec. gen. Genet.114, 31 (1971).
Herrlich, P., Schweiger, M.: T3 and T7 bacteriophage DNA-directed enzyme synthesisin vitro. J. Virol.6, 750 (1970).
Herrlich, P., Schweiger, M., Sauerbier, W.: Host- and phage-RNA polymerase mediated synthesis of T7 lysozymein vivo. Molec. gen. Genet.112, 152 (1971).
Scherzinger, E., Herrlich, P., Schweiger, M., Schuster, H.: The early region of the DNA of bacteriophage T7. Europ. J. Biochem.25, 341 (1972).
Schweiger, M., Gold, L. M.: DNA-dependentin vitro synthesis of bacteriophage enzymes. Cold Spr. Harb. Symp. quant. Biol.34, 763 (1969).
Schweiger, M., Gold, L. M.:E. coli andB. subtilis phage DNA-directed deoxycytidylate deaminase synthesis inE. coli extracts. J. biol. Chem.245, 5022 (1970).
Schweiger, M., Herrlich, P., Millette, R. L.: Gene expressionin vitro from DNA of bacteriophage T7. J. biol. Chem.246, 6707 (1971).
Seifert, W., Qasba, P., Walter, G., Palm, P., Schachner, M., Zillig, W.: Kinetics of the alteration and modification of DNA-dependent RNA-polymerase in T4-infectedE. coli cells. Europ. J. Biochem.9, 319 (1969).
Summers, W. C., Siegel, R. B.: Transcription of late phage RNA by T7 RNA polymerase. Nature (Lond.)228, 1160 (1970).
Zillig, W., Fuchs, E., Millette, R.: DNA-dependent RNA polymerase. In: G. L. Cantoni and D. R. Davies, Procedures in nucleic acid research, p. 323. New York: Harper & Row, Publ. 1966.
Anderson, E. S.: A rapid screening test for transfer factors in drug sensitive Enterobacteriaceae. Nature (Lond.)208, 1016–1017 (1965).
Anderson, E. S.: Influence of the Δ transfer factor on the phage sensitivity of salmonellae. Nature (Lond.)212, 795–799 (1966).
Anderson, E. S.: The ecology of transferable drug resistance in the enterobacteria. Ann. Rev. Microbiol.22, 131–180 (1968).
Anderson, E. S., Kelemen, M. V., Jones, C. M., Pitton, J.-S.: Study of the association of resistance to two drugs in a transferable determinant inSalmonella typhimurium. Genet. Res.11, 119–124 (1968).
Anderson, E. S., Lewis, M. J.: Characterisation of a transfer factor associated with drug resistance inSalmonella typhimurium. Nature (Lond.)208, 843–849 (1965).
Anderson, E. S., Mayhew, J. N., Grindley, N. D. F.: Transfer of a neomycin-kanamycin resistance determinant by the F factor ofEscherichia coli K12. Nature (Lond.)222, 349–351 (1969).
Easterling, S. B., Johnson, E. M., Wohlhieter, J. A., Baron, L. S.: Nature of lactose-fermentingSalmonella strains obtained from clinical sources. J. Bact.100, 35–41 (1969).
Egawa, R., Hirota, Y.: Inhibition of fertility by multiple drug-resistance factor (R) inEscherichia coli K12. Japan. J. Genet.37, 66–69 (1962).
Grindley, J. N., Anderson, E. S.: I-like resistance factors with thefi + character. Genet. Res.17, 267–271 (1971).
Grindley, N. D. F., Anderson, E. S., Smith, H. R., Grindley, J. N.: The effects ofSalmonella typhimurium on derepressed mutants of F-like factors. Genet. Res.17, 89–93 (1971).
Grindley, J. N., Grindley, N. D. F., Anderson, E. S.: Acridine treatment of F+ and Hfr strains ofEscherichia coli K12 carrying a neomycin-kanamycin resistance determinant. Genet. Res.15, 327–334 (1970).
Mäkelä, P. H., Lederberg, J., Lederberg, E. M.: Patterns of sexual recombination in enteric bacteria. Genetics47, 1427–1439 (1962).
Meynell, E., Datta, N.: Functional homology of the sex-factor and resistance transfer factors. Nature (Lond.)207, 884–885 (1965).
Scaife, J., Gross, J. D.: Inhibition of multiplication of an F-lac factor in Hfr cells ofEscherichia coli K-12. Biochem. biophys. Res. Commun.7, 403–407 (1962).
Smith, H. R., Grindley, J. N., Grindley, N. D. F., Anderson, E. S.: Derepression of F-lac inSalmonella typhimurium by a determinant for kanamycin resistance. Genet. Res.16, 349–353 (1970).
Watanabe, T., Fukasawa, T.: Episome-mediated transfer of drug resistance in Enterobacteriaceae. IV. Interactions between resistance transfer factor and F factor inEscherichia coli K12. J. Bact.83, 727–735 (1962).
Watanabe, T., Fukasawa, T., Takano, T.: Conversion of male bacteria ofEscherichia coli K12 to resistance to f phages by infection with the episome “resistance transfer factor”. Virology17, 218–219 (1962).
Author information
Authors and Affiliations
Additional information
Communicated by P. Starlinger
Experiments of Wetekam (accompanying paper) using heteroduplexes with markers in thegal operon ofE. coli gave analogous results.
Rights and permissions
About this article
Cite this article
Herrlich, P., Scherzinger, E., Schweiger, M. et al. Identification of the “sense”-strand of T7 DNA through heteroduplex directedin vitro enzyme synthesis. Molec. Gen. Genet. 118, 61–65 (1972). https://doi.org/10.1007/BF02428334
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02428334