Summary
The specificity ofin vitro transcription of T7 DNA byE. coli RNA polymerase was found to be remarkably influenced by the KCl concentration. At the lower KCl concentration, not onlyr-strand of early region but also late region andl-strand were transcribed. Such non-specific transcription was shown to be reduced by elevation of KCl concentration in the reaction mixture for RNA synthesis. At the higher KCl concentration than 0.2M, the RNA complementary tor-strand of early region was exclusively synthesized, the specificity of which is the same as the earlyin vivo transcription inE. coli infected with T7 phage.
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Communicated by H. Ozeki
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Matsukage, A. The effects of KCl concentration on the transcription byE. coli RNA polymerase. Molec. Gen. Genet. 118, 23–31 (1972). https://doi.org/10.1007/BF02428329
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DOI: https://doi.org/10.1007/BF02428329