Skip to main content
SpringerLink
Log in

The effects of KCl concentration on the transcription byE. coli RNA polymerase

I. Specific effect of the combination of nucleoside triphosphates

  • Published:
Molecular and General Genetics MGG Aims and scope Submit manuscript

Summary

The effect of KCl concentration on the RNA synthesis byE. coli RNA polymerase, especially on its initiation process, was compared using DNA's from ϕ80plac T7 and T4 phages as templates.

The KCl concentration showed different effect on the RNA synthesis depending on the DNA species used as a template. The reduction of RNA synthesis observed in the higher KCl concentrations was found to be caused by the decrease of initiation rate rather than caused by the decrease of elongation rate of RNA chain. The specific effect of the KCl concentration on the initiation rate depending on the species of DNA was observed only when the initiation complex was formed in the presence of a unique combination of three nucleoside triphosphates: ATP-GTP-CTP for T7 DNA or ATP-GTP-CTP and ATP-CTP-UTP for T4 DNA.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Anthony, D. D., Zeszotek, E., Goldthwait, D. A.: Initiation by the DNA-RNA-polymerase. Proc. nat. Acad. Sci.56, 1026 (1966).

    Article  PubMed  CAS  Google Scholar 

  • Bremer, H.: Influence of KCl on thein vitro transcription of T4 DNA. Cold Spr. Harb. Symp. quant. Biol.35, 109 (1970).

    CAS  Google Scholar 

  • Burgess, R. R., Travers, A. D., Dunn, J. J., Bautz, E. K. F.: Factor stimulating transcription by RNA polymerase. Nature (Lond.)221, 43 (1969).

    Article  CAS  Google Scholar 

  • Davis, R. W., Hyman, R. W.; Physical location of thein vitro RNA initiation site and termination sites of T7M DNA. Cold Spr. Harb. Symp. quant. Biol.35, 269 (1970).

    CAS  Google Scholar 

  • Crombrugghe, B. de, Chen, B., Gottesman, M., Pastan, I., Varmus, H. E., Emmer, M., Perlman, R. L.: Regulation oflac mRNA synthesis in a soluble cellfree system. Nature (Lond.) New Biol.230, 37 (1971).

    Google Scholar 

  • Goldthwait, D. A., Anthony, D. D., Wu, C. W.: Studies with the RNA polymerase. Lepetit Colloq. on RNA polymerase and transcription (ed. by L. Silvesti), p10. Amsterdam: North-Holland Publ. Co. 1970.

    Google Scholar 

  • Iida, Y., Kameyama, T., Ohshima, Y., Horiuchi, T.: Regulation of the lactose operonin vitro. I. Transcription of the lactose operon. Molec. gen. Genet.106, 296 (1970).

    Article  PubMed  CAS  Google Scholar 

  • Ishihama, A., Murakami, S., Fukuda, R., Matsukage, A., Kameyama, T.: The nature of initiation sites on DNA for the core RNA polymerase. Molec. gen. Genet.111, 66 (1971).

    Article  PubMed  CAS  Google Scholar 

  • Jones, O. W., Berg, P.: Studies on the binding of RNA-polymerase to polynucleotide. J. Molec. Biol.22, 199 (1966).

    Article  PubMed  CAS  Google Scholar 

  • Kameyama, T., Matsukage, A., Tanaka, Y.: Studies on the binding of RNA polymerase to DNA. J. cell Physiol.74, 219 (1969).

    Article  PubMed  CAS  Google Scholar 

  • Kawakami, H., Matsukage, A., Kameyama, T.: Purification ofE. coli RNA polymerase. Protein Nucleic Acid Enzyme14, 572 (1969).

    Google Scholar 

  • Maitra, U., Hurwitz, J.: The role of DNA in RNA synthesis, IX. Nucleoside triphosphate termini in RNA polymerase products. Proc. nat. Acad. Sci.54, 815 (1965).

    Article  PubMed  CAS  Google Scholar 

  • Matsukage, A.: The effect of KCl concentration on the transcription byE. coli RNA polymerase, II. Effect on the specificity of T7 transcription. Molec. gen. Genet.118, 23–31 (1972).

    PubMed  CAS  Google Scholar 

  • Matsukage, A., Murakami, S., Kameyama, T.: The isolation and characterisation of DNA region bound toE. coli RNA polimerase Biochim. Biophys. Acta (Anst.)179, 145 (1969).

    CAS  Google Scholar 

  • Richardson, J. P.: The binding of RNA polymerase to DNA. J. molec. Biol.21, 83 (1966).

    Article  PubMed  CAS  Google Scholar 

  • Sugiura, M., Okamoto, T., Takanami, M.: Starting nucleotide sequences of RNA synthesized on the replicative form DNA of coli phage fd. J. molec. Biol.43, 299 (1970).

    Article  Google Scholar 

  • Summers, W. C.: The process of infection with coliphage T7. I. Characterization of T7 RNA by polyacrylamide gel electrophoretic analisis. Virology39, 175 (1969).

    Article  PubMed  CAS  Google Scholar 

  • Summers, W. C., Siegel, R. B.: Regulation of coliphage T7 RNA metabolismin vivo andin vitro. Cold Spr. Harb. Symp. quant. Biol.35, 253 (1970).

    CAS  Google Scholar 

  • Takanami, M., Okamoto, T., Sugiura, M.: The starting nucleotide sequences and size of RNA transcribedin vitro on phage DNA templates. Cold Spr. Harb. Symp. quant. Biol.35, 179 (1970).

    CAS  Google Scholar 

  • Travers, A., Kamen, R., Cahel, M.: Thein vitro synthesis of ribosomal RNA. Cold. Spr. Harb. Symp. quant. Biol.35, 415 (1970).

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Molecular Biology, Cancer Research Institute, Kanazawa University, Kanazawa, Japan

    A. Matsukage

  2. Biosynthesis Section Laboratory of Biochemistry National Cancer Institute, National Institute of Health, 20014, Bethesda, Maryland, USA

    A. Matsukage

Authors
  1. A. Matsukage
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by H. Ozeki

Rights and permissions

Reprints and permissions

About this article

Cite this article

Matsukage, A. The effects of KCl concentration on the transcription byE. coli RNA polymerase. Molec. Gen. Genet. 118, 11–22 (1972). https://doi.org/10.1007/BF02428328

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02428328

Keywords

Search

Navigation