Summary
In a merodiploid strain ofEscherichia coli heterozygous for the ribosomal protein genesspc andstr, deletions were observed preventing the expression of either gene but permitting the expression of the other. This suggests that thespc andstr genes are in separate transcriptional units.
Similar content being viewed by others
References
Anderson, P., Jr. Sensitivity and resistance to spectinomycin inEscherichia coli. J. Bact.100, 939–947 (1969)
Bastarrachea, F., Tam, E., Gonzáles, A.: Dominance of streptomycin sensitivity over dependence inEscherichia coli K12 merodiploids. Genetics63, 759–774 (1969)
Cabezón, T., Faelen, M., Wilde, M. de, Bollen, A., Thomas, R.: Expression of ribosomal protein genes inEscherichia coli. Molec. gen. Genet.137, 125–129 (1975)
Davies, J., Nomura, M.: The genetics of bacterial ribosomes. Ann. Rev. Genet.6, 203–234 (1972)
Dennis, P.: Synthesis of individual ribosomal proteins inEscherichia coli B/r. J. molec. Biol.89, 223–232 (1974)
Hirsch-Kauffmann, M., Schweiger, M., Herrlich, P., Ponta, H., Rahmsdorf, H.J., Pai, S.H., Wittmann, H.G.: Transcriptional units for ribosomal proteins ofEscherichia coli. Europ. J. Biochem. 52, 469–474 (1975)
Jaskunas, S.R., Nomura, M., Davies, J.: Genetics of bacterial ribosomes. In: Ribosomes (Nomura, M., Tissières, A., Lengyel, P. eds.), p. 333–368. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory 1974
Jaskunas, S.R., Lindahl, L., Nomura, M.: Specialized transducing phages for ribosomal protein genes ofEscherichia coli. Proc. nat. Acad. Sci. (Wash.)72, 6–10 (1975a)
Jaskunas, S.R., Lindahl, L., Nomura, M.: Isolation of polar insertion mutants and the direction of transcription of ribosomal protein genes inE. coli. Nature (Lond.)256, 183–187 (1975b)
Jaskunas, S.R., Lindahl, L., Nomura, M., Burgess, R.R.: Identification of two copies of the gene for the elongation factor EF-Tu inE. coli. Nature (Lond.)257, 458–462 (1975)
Lindahl, L., Jaskunas, S.R., Dennis, P., Nomura, M.: Cluster of genes inEscherichia coli for ribosomal proteins, ribosomal RNA, and RNA polymerase subunits. Proc. nat. Acad. SSci. (Wash.)72, 2743–2747 (1975)
Low, B.: Formation of merodiploids in matings with a class of Rec− recipient strains ofEscherichia coli K12. Proc. nat. Acad. Sci. (Wash.)60, 160–167 (1968)
Luria, S.E., Burrous, J.W.: Hybridization betweenEscherichia coli andShigella. J. Bact.74, 461–476 (1957)
Miller, J.: Experiments in molecular genetics p. 82–83 New York: Cold Spring Harbor Laboratory 1972.
Molin, S., Meyenburg, K. von, Gulløv, K., Maaløe, O.: The size of transcriptional units for ribosomal proteins inEscherichia coli. Molec. gen. Genet.129, 11–26 (1974)
Monod, J., Cohen-Bazier, G., Cohn, M.: Sur la biosynthèse de la β-galactosidase (lactose) chezEscherichia coli. La spécificité de l'induction. Biochim. biophys. Acta (Amst.)7, 758–779 (1951)
Newcombe, H.B., Hawirko, R.: Spontaneous mutation to streptomycin resistance and dependence inEscherichia coli. J. Bact.57, 565–572 (1949)
Nomura, M., Engbaek, F.: Expression of ribosomal protein genes as analyzed by bacteriophage Mu-induced mutations. Proc. nat. Acad. Sci. (Wash.)69, 1526–1530 (1972)
Osawa, S., Otaka, E., Takata, R., Dekio, S., Matsubara, M., Itoh, M., Muto, M., Tanaka, K., Teraoka, Tamaki, M.: Ribosomal protein genes in bacteria. In: Functional units in protein synthesis, (Cox, R.A. and Hadjiolov, A.A., eds.), p. 313–336. London: Academic Press 1972
Pittard, J., Wallace, B.J.: Distribution and function of genes concerned with aromatic biosynthesis inEscherichia coli. J. Bact.91, 1494–1508 (1966)
Sparling, P.F., Modolell, J., Takeda, Y., Davis, B.: Ribosomes fromEscherichia coli merodiploids heterozygous for resistance to streptomycin and to spectinomycin. J. molec. Biol.37, 407–421 (1968)
Watson, R.J., Parker, J., Fiil, N.P., Flaks, J.G., Friesen, J.: New chromosomal location for structural genes of ribosomal proteins. Proc. nat. Acad. Sci. (Wash.)72, 2765–2769 (1975)
Wilde, M. de, Michel, F., Broman, K.: The structural gene for ribosomal protein S18 inEscherichia coli III. Mapping outside the ribosomal protein gene cluster at minute 84 on the genome. Molec. gen. Genet.133, 329–333 (1974)
Author information
Authors and Affiliations
Additional information
Communicated by H.G. Wittmann
Rights and permissions
About this article
Cite this article
Andrésson, Ó.S., Magnúsdóttir, R.Á. & Eggertsson, G. Deletions of ribosomal protein genes inEscherichia coli merodiploids heterozygous for resistance to streptomycin and spectinomycin. Molec. Gen. Genet. 144, 127–130 (1976). https://doi.org/10.1007/BF02428100
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02428100