Summary
The DNA sequences upstream of the 5S RNA genes inNeurospora crassa are largely different from one another, but share a short consensus sequence located in the segment 29 to 26 nucleotides preceding the transcribed region. Differences among flanking sequences do not appear to affect transcription. Deletion analysis indicates, however, that a DNA segment including the conserved “TATA box” is required for in vitro transcription of Neurospora 5S RNA genes.
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Communicated by W. Gajewski
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Selker, E.U., Morzycka-Wroblewska, E., Stevens, J.N. et al. An upstream signal is required for in vitro transcription ofNeurospora 5S RNA genes. Molec. Gen. Genet. 205, 189–192 (1986). https://doi.org/10.1007/BF02428052
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DOI: https://doi.org/10.1007/BF02428052