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Repeated sequences with unusual properties from the divergent region ofCrithidia oncopelti maxicircle kinetoplast DNA

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Summary

The occurrence of bacterial promoter-like sequences was shown in the divergent region ofCrithidia oncopelti maxicircular kinetoplast DNA. A promoter-cloning vector based on the neomycin phosphotransferase II (NPT II) gene was used to localize promoters in three homologous blocks of repeated sequences. The elements found displayed greater promoter strength inEscherichia coli than the normal promoter of the NPT II gene. Sequences of three promoter-containing inserts from different blocks were determined and typical prokaryotic promoter sequences were localized.

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Abbreviations

bp:

base pairs

mtDNA:

mitochondrial DNA

kb:

kilobase pairs

kDNA:

kinetoplast DNA

MkDNA:

maxicircle kinetoplast DNA

SDS:

sodium dodecyl sulphate

SSC:

150 mM

NaCl:

15 mM Na citrate

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Authors and Affiliations

  1. Biology Dept., Moscow State University, Chair of Molecular Biology, 117234, Moscow, USSR

    Ivan A. Tarassoff, Evgeny V. Kuzmin, Igor V. Levchenko & Galina N. Zaitseva

Authors
  1. Ivan A. Tarassoff
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  2. Evgeny V. Kuzmin
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  3. Igor V. Levchenko
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  4. Galina N. Zaitseva
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Communicated by D. Goldfarb

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Tarassoff, I.A., Kuzmin, E.V., Levchenko, I.V. et al. Repeated sequences with unusual properties from the divergent region ofCrithidia oncopelti maxicircle kinetoplast DNA. Molec. Gen. Genet. 205, 122–126 (1986). https://doi.org/10.1007/BF02428041

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  • DOI: https://doi.org/10.1007/BF02428041

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