Summary
We have characterized severalMyxococcus xanthus mutants in which carotenoid synthesis is affected. Six of them produce carotenoids in the absence of visible light, an absolute requirement for carotenogenesis in wild-type strains, and thus will be referred to as constitutive mutants. The six corresponding mutations have been mapped by transductional analysis mediated by linked Tn5 insertions. Five of the mutations have been localized to a single locus, closely linked to Tn5 insertion ΩMR136 and loosely linked to ΩDK4611. The sixth mutation, present in strain MR7, is linked to Tn5 insertion ΩMR134. Another Tn5 insertion site (ΩDK2836) has been characterized and found to be linked to the MR7 colour mutation and to ΩMR134. Darkor light-grown cultures of strains carrying the Tn5 insertion ΩDK2836 do not produce carotenoids even if they simultaneously carry any of the constitutive mutations.
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References
Avery L, Kaiser D (1983a) In situ transposon replacement and isolation of a spontaneous tandem genetic duplication. Mol Gen Genet 191: 99–109
Avery L, Kaiser D (1983b) Construction of tandem genetic duplications with defined endpoints inMyxococcus xanthus. Mol Gen Genet 191: 110–117
Blackhart BD, Zusman DR (1985) Cloning and complementation analysis of the “frizzy” genes ofMyxococcus xanthus. Mol Gen Genet 198: 243–254
Bretscher AP, Kaiser D (1978) Nutrition ofMyxococcus xanthus, a fruiting Myxobacterium. J Bacteriol 133: 763–768
Burchard RP, Dworkin M (1966) Light-induced lysis and carotenogenesis inMyxococcus xanthus. J Bacteriol 91: 535–545
Burchard RP, Hendricks SB (1969) Actium spectrum for carotenogenesis inMyxococcus xanthus. J Bacteriol 91: 535–545
Burchard RP, Hendricks SB (1969) Actium spectrum for carotenogenesis inMyxococcus xanthus. J Bacteriol 97: 1165–1168
Burchard RP, Burchard AC, Parish JH (1977) Pigmentation phenotype instability inMyxococcus xanthus. Can J Microbiol 23: 1657–1662
Campos JM, Geisselsoder J, Zusman DR (1978) Isolation of bacteriophage Mx4, a generalized transducing phage forMyxococcus xanthus. J Mol Biol 119: 167–178
Cerdá-Olmedo E, Torres-Martínez S (1979) Genetics and regulation of carotene biosynthesis. Pure Appl Chem 51: 631–637
Goodwin TW (1980) The biochemistry of the carotenoids. Chapman and Hall, London
Hagen DC, Bretscher AP, Kaiser D (1978) Synergism between morphogenetic mutants ofMyxococcus xanthus. Dev Biol 64: 284–296
Kaiser D, Manoil C, Dworkin M (1979) Myxobacteria: Cell interactions, genetics, and development. Annu Rev Microbiol 33: 595–639
Kleinig H (1974) Inhibition of carotenoid synthesis inMyxococcus fulvus (Myxobacterales). Arch Microbiol 97: 217–226
Kleinig H (1975) On the utilization in vivo of lycopene and phytoene as precursors for the formation of carotenoid glucoside ester and on the regulation of carotenoid biosynthesis inMyxococcus fulvus. Eur J Biochem 57: 301–308
Kleinig H, Reichenbach H (1973) Biosynthesis of carotenoid glucoside esters inMyxococcus fulvus (Myxobacterales): Inhibition by nicotine and carotenoid turnover. Biochem Biophys Acta 306: 249–256
Kroos L, Kaiser D (1983) Construction of Tn5 lac, a transposon that fuseslacZ expression to exogenous promoter, and its introduction intoMyxococcus xanthus. Proc Natl Acad Sci USA 81: 5816–5820
Kuner JM, Kaiser D (1981) Introduction of transposon Tn5 intoMyxococcus for analysis of development and other nonselectable mutants. Proc Natl Acad Sci USA 78: 425–429
Kuner JM, Avery L, Berg D, Kaiser D (1981) Use of transposon Tn5 in the genetic analysis ofMyxococcus xanthus. In: Schlessinger D (ed) Microbiology 1981, American Society for Microbiology, Washington DC, pp 128–132
Martin S, Sodergren E, Masuda T, Kaiser D (1978) Systematic isolation of transducing phages forMyxococcus xanthus. Virology 88: 44–53
Murillo FJ, Cerdá-Olmedo E (1976) Regulation of carotene synthesis inPhycomyces. Mol Gen Genet 148: 19–24
Reichenbach H, Kleinig H (1984) Pigments of myxobacteria. In: Rosenberg E (ed) Myxobacteria: Development and cell interactions. Springer, New York Berlin Heidelberg, pp 127–137
Rosenberg E (ed) (1984) Myxobacteria: Development and cell interactions. Springer, New York Berlin Heidelberg
Ruiz-Vázquez R, Murillo FJ (1984) Abnormal motility and fruiting behavior ofMyxococcus xanthus bacteriophage-resistant strains induced by a clear-plaque mutant of bacteriophage Mx8. J Bacteriol 160: 818–821
Shimkets L, Gill R, Kaiser D (1983) Developmental cell interactions inMyxococcus xanthus and theSpoC locus. Proc Natl Acad Sci USA 80: 1406–1410
Sodergren E, Kaiser D (1983) Insertions of Tn5 near genes that govern stimulatable cell motility inMyxococcus. J Mol Biol 167: 295–310
Wu TT (1966) A model for three-point analysis of random general transduction. Genetics 54: 405–410
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Martínez-Laborda, A., Elías, M., Ruiz-Vázquez, R. et al. Insertions of Tn5 linked to mutations affecting carotenoid synthesis inMyxococcus xanthus . Molec. Gen. Genet. 205, 107–114 (1986). https://doi.org/10.1007/BF02428039
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DOI: https://doi.org/10.1007/BF02428039