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T-DNA structure in transgenic tobacco plants with multiple independent integration sites

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Summary

Transgenic tobacco plants were produced by inoculation of leaf disks withAgrobacterium tumefaciens harboring a disarmed binary vector containing soybean leghemoglobin Lbc3 and glycinin G2 genes. Physical and genetic characterization of these plants indicated that one to six copies of DNA from the vector were transferred and maintained in the plant genome. Approximately 30% of the copies transferred were found to be incomplete or rearranged and in some cases joined as inverted repeats. The transferred DNA was found at multiple genetic loci in five of the six cases examined. In one plant, kanamycin-resistance traits were at four independent chromosomal positions, although two were genetically linked at about 3 centimorgans. Thus,Agrobacterium-mediated DNA transfer to plants has some characteristics in common with “natural” systems in animals, such as retroviral or P-element derived systems, some characteristics in common with “artificial” systems, such as microinjection, electroporation, or calcium phosphate coprecipitation techniques, and some novel characteristics.

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Authors and Affiliations

  1. Molecular Biology Group, ARCO Plant Cell Research Institute, 6560 Trinity Ct., 94568, Dublin, CA, USA

    Albert Spielmann & Robert B. Simpson

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  1. Albert Spielmann
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  2. Robert B. Simpson
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Communicated by R.B. Goldberg

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Spielmann, A., Simpson, R.B. T-DNA structure in transgenic tobacco plants with multiple independent integration sites. Molec. Gen. Genet. 205, 34–41 (1986). https://doi.org/10.1007/BF02428030

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  • DOI: https://doi.org/10.1007/BF02428030

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